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Molecules 2016, 21(9), 1192; doi:10.3390/molecules21091192

Effects of Culture Substrate Made of Poly(N-isopropylacrylamide-co-acrylic acid) Microgels on Osteogenic Differentiation of Mesenchymal Stem Cells

1
Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
2
Ministry of Education Key Laboratory for Regenerative Medicine, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Chih-Chang Chu
Received: 12 July 2016 / Revised: 23 August 2016 / Accepted: 30 August 2016 / Published: 9 September 2016
(This article belongs to the Special Issue Stimuli-Responsive Biomaterials in Biomedical Applications)
View Full-Text   |   Download PDF [9446 KB, uploaded 9 September 2016]   |  

Abstract

Poly(N-isopropylacrylamide) (PNIPAM)-based polymers and gels are widely known and studied for their thermoresponsive property. In the biomaterials category, they are regarded as a potential cell culture substrate, not only because of their biocompatibility, but also their special character of allowing controlled detachment of cells via temperature stimulus. Previous research about PNIPAM-based substrates mostly concentrated on their effects in cell adhesion and proliferation. In this study, however, we investigate the influence of the PNIPAM-based substrate on the differentiation capacity of stem cells. Especially, we choose P(NIPAM-AA) microgels as a culture dish coating and mesenchymal stem cells (MSCs) are cultured on top of the microgels. Interestingly, we find that the morphology of MSCs changes remarkably on a microgel-coated surface, from the original spindle form to a more stretched and elongated cell shape. Accompanied by the alternation in morphology, the expression of several osteogenesis-related genes is elevated even without inducing factors. In the presence of full osteogenic medium, MSCs on a microgel substrate show an enhancement in the expression level of osteopontin and alizarin red staining signals, indicating the physical property of substrate has a direct effect on MSCs differentiation. View Full-Text
Keywords: microgels; p(N-isopropylacrylamide-co-acrylic acid); mesenchymal stem cells; extracellular matrix; cell morphology; osteogenesis microgels; p(N-isopropylacrylamide-co-acrylic acid); mesenchymal stem cells; extracellular matrix; cell morphology; osteogenesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Dai, Z.; Shu, Y.; Wan, C.; Wu, C. Effects of Culture Substrate Made of Poly(N-isopropylacrylamide-co-acrylic acid) Microgels on Osteogenic Differentiation of Mesenchymal Stem Cells. Molecules 2016, 21, 1192.

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