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Molecules 2016, 21(8), 1103; doi:10.3390/molecules21081103

Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection

Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China
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Academic Editor: Chih-Chang Chu
Received: 30 July 2016 / Revised: 15 August 2016 / Accepted: 18 August 2016 / Published: 20 August 2016
(This article belongs to the Special Issue Stimuli-Responsive Biomaterials in Biomedical Applications)
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Abstract

Endo/lysosomal escape and subsequent nuclear translocation are recognized as the two major challenges for efficient gene transfection. Herein, nuclear localization signal (NLS) peptide sequences and oligomeric lysine sequences were crosslinked via disulfide bonds to obtain glutathione (GSH) reducible polypeptide (pNLS). The pNLS could condense DNA into compact positive-charged complexes with redox sensitivity, and then gold nanoclusters (AuNC) were further decorated to the surface via electrostatic interactions obtaining versatile pNLS/DNA/AuNC complexes. The AuNC could generate reactive oxygen species (ROS) under NIR-irradiation and accelerate the endo/lysosomal escape of the complexes, and then the pNLS sequence degraded by GSH in cytoplasm would release the DNA and facilitate the subsequent nuclear translocation for enhanced gene transfection. View Full-Text
Keywords: NIR-responsive; redox-responsive; nuclear localization signal; endo/lysosomal escape; gene delivery NIR-responsive; redox-responsive; nuclear localization signal; endo/lysosomal escape; gene delivery
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Lei, Q.; Hu, J.-J.; Rong, L.; Cheng, H.; Sun, Y.-X.; Zhang, X.-Z. Gold Nanocluster Decorated Polypeptide/DNA Complexes for NIR Light and Redox Dual-Responsive Gene Transfection. Molecules 2016, 21, 1103.

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