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Molecules 2016, 21(9), 1119; doi:10.3390/molecules21091119

Quantification of Oxidized and Unsaturated Bile Alcohols in Sea Lamprey Tissues by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

Department of Fisheries and Wildlife, Michigan State University, East Lansing, MI 48824, USA
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Academic Editor: Derek J. McPhee
Received: 18 July 2016 / Revised: 20 August 2016 / Accepted: 22 August 2016 / Published: 24 August 2016
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Abstract

A sensitive and reliable method was developed and validated for the determination of unsaturated bile alcohols in sea lamprey tissues using liquid-liquid extraction and ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The liver, kidney, and intestine samples were extracted with acetonitrile and defatted by n-hexane. Gradient UHPLC separation was performed using an Acquity BEH C18 column with a mobile phase of water and methanol containing 20 mM triethylamine. Multiple reaction monitoring modes of precursor-product ion transitions for each analyte was used. This method displayed good linearity, with correlation coefficients greater than 0.99, and was validated. Precision and accuracy (RSD %) were in the range of 0.31%–5.28%, while mean recoveries were between 84.3%–96.3%. With this technique, sea lamprey tissue samples were analyzed for unsaturated bile alcohol analytes. This method is practical and particularly suitable for widespread putative pheromone residue analysis. View Full-Text
Keywords: metabolites; sulfated bile alcohol; UPLC-MS/MS; fish metabolites; sulfated bile alcohol; UPLC-MS/MS; fish
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MDPI and ACS Style

Li, K.; Scott, A.M.; Chung-Davidson, Y.-W.; Bussy, U.; Patel, T.; Middleton, Z.E.; Li, W. Quantification of Oxidized and Unsaturated Bile Alcohols in Sea Lamprey Tissues by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry. Molecules 2016, 21, 1119.

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