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Molecules 2016, 21(8), 1071; doi:10.3390/molecules21081071

Neuroprotective Effects of Methyl 3,4-Dihydroxybenzoate against TBHP-Induced Oxidative Damage in SH-SY5Y Cells

1
Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China
2
School of International Education, Anhui Medical University, Hefei 230000, China
3
School of Nursing, Guangdong Pharmaceutical University, Guangzhou 510632, China
4
The First Affiliated Hospital of Jinan University, Guangzhou 510632, China
5
Department of Pathogen Biology and Medical Immunology, School of Basic Medicine, Ningxia Medical University, Yinchuan 750021, China
6
Department of Anatomy, Li Ka Shing Faculty of Medicine of The University of Hong Kong, Hong Kong 999077, China
7
Institute of Brain Sciences, Jinan University, Guangzhou 510632, China
*
Author to whom correspondence should be addressed.
Academic Editors: Luciano Saso, László Dux, Grzegorz Wegrzyn and Tamás Csont
Received: 29 June 2016 / Revised: 8 August 2016 / Accepted: 10 August 2016 / Published: 22 August 2016
(This article belongs to the Special Issue Chemistry and Pharmacology of Modulators of Oxidative Stress)
View Full-Text   |   Download PDF [3027 KB, uploaded 22 August 2016]   |  

Abstract

This study investigated the neuroprotective effects of methyl 3,4-dihydroxybenzoate (MDHB) against t-butyl hydroperoxide (TBHP) induced oxidative damage in SH-SY5Y (human neuroblastoma cells) and the underlying mechanisms. SH-SY5Y were cultured in DMEM + 10% FBS for 24 h and pretreated with different concentrations of MDHB or N-acetyl-l-cysteine (NAC) for 4 h prior to the addition of 40 μM TBHP for 24 h. Cell viability was analyzed using the methylthiazolyltetrazolium (MTT) and lactate dehydrogenase (LDH) assays. An annexin V-FITC assay was used to detect cell apoptosis rates. The 2′,7′-dichlorofluorescin diacetate (DCFH-DA) assay was used to determine intracellular ROS levels. The activities of antioxidative enzymes (GSH-Px and SOD) were measured using commercially available kits. The oxidative DNA damage marker 8-OHdG was detected using ELISA. Western blotting was used to determine the expression of Bcl-2, Bax, caspase 3, p-Akt and Akt proteins in treated SH-SY5Y cells. Our results showed that MDHB is an effective neuroprotective compound that can mitigate oxidative stress and inhibit apoptosis in SH-SY5Y cells. View Full-Text
Keywords: methyl 3,4-dihydroxybenzoate; oxidative stress; apoptosis; neuroprotection; nuclear factor erythroid 2-related factor 2 methyl 3,4-dihydroxybenzoate; oxidative stress; apoptosis; neuroprotection; nuclear factor erythroid 2-related factor 2
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cai, L.; Wang, L.-F.; Pan, J.-P.; Mi, X.-N.; Zhang, Z.; Geng, H.-J.; Wang, J.-H.; Hu, S.-H.; Zhang, W.; Gao, Q.; Wu, W.-T.; Luo, H.-M. Neuroprotective Effects of Methyl 3,4-Dihydroxybenzoate against TBHP-Induced Oxidative Damage in SH-SY5Y Cells. Molecules 2016, 21, 1071.

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