Next Article in Journal
Metabolomics Provides Quality Characterization of Commercial Gochujang (Fermented Pepper Paste)
Previous Article in Journal
Exploring Flow Procedures for Diazonium Formation
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(7), 920; doi:10.3390/molecules21070920

DNA Cleavage and Condensation Activities of Mono- and Binuclear Hybrid Complexes and Regulation by Graphene Oxide

1
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China
2
School of Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China
3
Jiangsu Key Laboratory of Target Drug and Clinical Application, Xuzhou Medical College, Xuzhou 221004, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 7 June 2016 / Revised: 26 June 2016 / Accepted: 8 July 2016 / Published: 15 July 2016
View Full-Text   |   Download PDF [4739 KB, uploaded 15 July 2016]   |  

Abstract

Hybrid complexes with N,N′-bis(2-benzimidazolylmethyl)amine and cyclen moieties are novel enzyme mimics and controlled DNA release materials, which could interact with DNA through three models under different conditions. In this paper, the interactions between plasmid DNA and seven different complexes were investigated, and the methods to change the interaction patterns by graphene oxide (GO) or concentrations were also investigated. The cleavage of pUC19 DNA promoted by target complexes were via hydrolytic or oxidative mechanisms at low concentrations ranging from 3.13 × 10−7 to 6.25 × 10−5 mol/L. Dinuclear complexes 2a and 2b can promote the cleavage of plasmid pUC19 DNA to a linear form at pH values below 7.0. Furthermore, binuclear hybrid complexes could condense DNA as nanoparticles above 3.13 × 10−5 mol/L and partly release DNA by graphene oxide with π-π stacking. Meanwhile, the results also reflected that graphene oxide could prevent DNA from breaking down. Cell viability assays showed dinuclear complexes were safe to normal human hepatic cells at relative high concentrations. The present work might help to develop novel strategies for the design and synthesis of DNA controllable releasing agents, which may be applied to gene delivery and also to exploit the new application for GO. View Full-Text
Keywords: cyclen; N,N′-bis(2-benzimidazolylmethyl)amine; DNA condenstion; DNA release; enzyme mimic; graphene oxide cyclen; N,N′-bis(2-benzimidazolylmethyl)amine; DNA condenstion; DNA release; enzyme mimic; graphene oxide
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Li, S.; Dai, M.; Zhang, C.; Jiang, B.; Xu, J.; Zhou, D.; Gu, Z. DNA Cleavage and Condensation Activities of Mono- and Binuclear Hybrid Complexes and Regulation by Graphene Oxide. Molecules 2016, 21, 920.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top