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Molecules 2016, 21(7), 902; doi:10.3390/molecules21070902

In Situ Enzymatically Generated Photoswitchable Oxidase Mimetics and Their Application for Colorimetric Detection of Glucose Oxidase

The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi 214122, China
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Academic Editors: Hui Wei and Derek J. Mcphee
Received: 30 May 2016 / Revised: 26 June 2016 / Accepted: 7 July 2016 / Published: 9 July 2016
(This article belongs to the Special Issue Nanozymes and Beyond)
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Abstract

In this study, a simple and amplified colorimetric assay is developed for the detection of the enzymatic activity of glucose oxidase (GOx) based on in situ formation of a photoswitchable oxidase mimetic of PO43−-capped CdS quantum dots (QDs). GOx catalyzes the oxidation of 1-thio-β-d-glucose to give 1-thio-β-d-gluconic acid which spontaneously hydrolyzes to β-d-gluconic acid and H2S; the generated H2S instantly reacts with Cd2+ in the presence of Na3PO4 to give PO43−-stabilized CdS QDs in situ. Under visible-light (λ ≥ 400 nm) stimulation, the PO43−-capped CdS QDs are a new style of oxidase mimic derived by producing some active species, such as h+, OH, O2•− and a little H2O2, which can oxidize the typical substrate (3,3,5,5-tetramethylbenzydine (TMB)) with a color change. Based on the GOx-triggered growth of the oxidase mimetics of PO43−-capped CdS QDs in situ, we developed a simple and amplified colorimetric assay to probe the enzymatic activity of GOx. The proposed method allowed the detection of the enzymatic activity of GOx over the range from 25 μg/L to 50 mg/L with a low detection limit of 6.6 μg/L. We believe the PO43−-capped CdS QDs generated in situ with photo-stimulated enzyme-mimicking activity may find wide potential applications in biosensors. View Full-Text
Keywords: glucose oxidase; photoswitchable oxidase mimetics; visible light; colorimetric sensor glucose oxidase; photoswitchable oxidase mimetics; visible light; colorimetric sensor
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Cao, G.-X.; Wu, X.-M.; Dong, Y.-M.; Li, Z.-J.; Wang, G.-L. In Situ Enzymatically Generated Photoswitchable Oxidase Mimetics and Their Application for Colorimetric Detection of Glucose Oxidase. Molecules 2016, 21, 902.

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