Location and Effects of an Antitumoral Catechin on the Structural Properties of Phosphatidylethanolamine Membranes
AbstractGreen tea catechins exhibit high diversity of biological effects including antioncogenic properties, and there is enormous interest in their potential use in the treatment of a number of pathologies. It is recognized that the mechanism underlying the activity of catechins relay in part in processes related to the membrane, and many studies revealed that the ability of catechins to interact with lipids plays a probably necessary role in their mechanism of action. We present in this work the characterization of the interaction between an antitumoral synthetically modified catechin (3-O-(3,4,5-trimethoxybenzoyl)-(−)-catechin, TMCG) and dimiristoylphosphatidyl-ethanolamine (DMPE) membranes using an array of biophysical techniques which include differential scanning calorimetry, X-ray diffraction, infrared spectroscopy, atomic force microscopy, and molecular dynamics simulations. We found that TMCG incorporate into DMPE bilayers perturbing the thermotropic transition from the gel to the fluid state forming enriched domains which separated into different gel phases. TMCG does not influence the overall bilayer assembly of phosphatidylethanolamine systems but it manages to influence the interfacial region of the membrane and slightly decrease the interlamellar repeat distance of the bilayer. TMCG seems to be located in the interior of the phosphatidylethanolamine bilayer with the methoxy groups being in the deepest position and some portion of the molecule interacting with the water interface. We believe that the reported interactions are significant not only from the point of view of the known antitumoral effect of TMCG, but also might contribute to understanding the basic molecular mechanism of the biological effects of the catechins found at the membrane level. View Full-Text
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Casado, F.; Teruel, J.A.; Casado, S.; Ortiz, A.; Rodríguez-López, J.N.; Aranda, F.J. Location and Effects of an Antitumoral Catechin on the Structural Properties of Phosphatidylethanolamine Membranes. Molecules 2016, 21, 829.
Casado F, Teruel JA, Casado S, Ortiz A, Rodríguez-López JN, Aranda FJ. Location and Effects of an Antitumoral Catechin on the Structural Properties of Phosphatidylethanolamine Membranes. Molecules. 2016; 21(7):829.Chicago/Turabian Style
Casado, Francisco; Teruel, José A.; Casado, Santiago; Ortiz, Antonio; Rodríguez-López, José N.; Aranda, Francisco J. 2016. "Location and Effects of an Antitumoral Catechin on the Structural Properties of Phosphatidylethanolamine Membranes." Molecules 21, no. 7: 829.
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