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Molecules 2016, 21(9), 1186; doi:10.3390/molecules21091186

Catechins Variously Affect Activities of Conjugation Enzymes in Proliferating and Differentiated Caco-2 Cells

1
Department of Biochemical Sciences, Faculty of Pharmacy, Charles University, Hradec Králové CZ-50005, Czech Republic
2
Toxicogenomics Unit, Centre of Toxicology and Health Safety, National Institute of Public Health, Prague CZ-10042, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Joshua D. Lambert
Received: 27 July 2016 / Revised: 24 August 2016 / Accepted: 1 September 2016 / Published: 7 September 2016
(This article belongs to the Special Issue Catechins and Human Health: Current State of the Science)
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Abstract

The knowledge of processes in intestinal cells is essential, as most xenobiotics come into contact with the small intestine first. Caco-2 cells are human colorectal adenocarcinoma that once differentiated, exhibit enterocyte-like characteristics. Our study compares activities and expressions of important conjugation enzymes and their modulation by green tea extract (GTE) and epigallocatechin gallate (EGCG) using both proliferating (P) and differentiated (D) caco-2 cells. The mRNA levels of the main conjugation enzymes were significantly elevated after the differentiation of Caco-2 cells. However, no increase in conjugation enzymes’ activities in differentiated cells was detected in comparison to proliferating ones. GTE/EGCG treatment did not affect the mRNA levels of any of the conjugation enzymes tested in either type of cells. Concerning conjugation enzymes activities, GTE/EGCG treatment elevated glutathione S-transferase (GST) activity by approx. 30% and inhibited catechol-O-methyltransferase (COMT) activity by approx. 20% in differentiated cells. On the other hand, GTE as well as EGCG treatment did not significantly affect the activities of conjugation enzymes in proliferating cells. Administration of GTE/EGCG mediated only mild changes of GST and COMT activities in enterocyte-like cells, indicating a low risk of GTE/EGCG interactions with concomitantly administered drugs. However, a considerable chemo-protective effect of GTE via the pronounced induction of detoxifying enzymes cannot be expected as well. View Full-Text
Keywords: green tea extract; epigallocatechin gallate; sulfotransferase; glutathione S-transferase; catechol-O-methyltransferase; UDP-glucuronosyl transferase; enterocyte-like cells; cancer cells green tea extract; epigallocatechin gallate; sulfotransferase; glutathione S-transferase; catechol-O-methyltransferase; UDP-glucuronosyl transferase; enterocyte-like cells; cancer cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lněničková, K.; Procházková, E.; Skálová, L.; Matoušková, P.; Bártíková, H.; Souček, P.; Szotáková, B. Catechins Variously Affect Activities of Conjugation Enzymes in Proliferating and Differentiated Caco-2 Cells. Molecules 2016, 21, 1186.

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