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Molecules 2016, 21(6), 741; doi:10.3390/molecules21060741

Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability

1
National & Local United Engineering Lab of Quality Controlling Technology and Instrumentation for Marine Food, China Jiliang University, Hangzhou 310018, China
2
Department of Food Science, Ocean College, Zhejiang University of Technology, Hangzhou 310014, China
3
Inspection and Quarantine Center of Shandong Exit & Entry Inspection and Quarantine Burean, Qingdao 266002, China
4
Ningbo Inspection and Quarantine Institute of Science and Technology, Ningbo 315000, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Didier Astruc
Received: 31 March 2016 / Revised: 25 May 2016 / Accepted: 31 May 2016 / Published: 8 June 2016
(This article belongs to the Collection Nanomedicine)
View Full-Text   |   Download PDF [3658 KB, uploaded 8 June 2016]   |  

Abstract

The main purpose of this study was to optimize the preparation of lysozyme nanoliposomes using response surface methodology and measure their stability. The stabilities of lysozyme nanoliposomes in simulated gastrointestinal fluid (SGF), simulated intestinal fluid (SIF), as well as pH, temperature and sonication treatment time were evaluated. Reverse-phase evaporation method is an easy, speedy, and beneficial approach for nanoliposomes’ preparation and optimization. The optimal preparative conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 3.86, lysozyme concentration of 1.96 mg/mL, magnetic stirring time of 40.61 min, and ultrasound time of 14.15 min. At the optimal point, encapsulation efficiency and particle size were found to be 75.36% ± 3.20% and 245.6 nm ± 5.2 nm, respectively. The lysozyme nanoliposomes demonstrated certain stability in SGF and SIF at a temperature of 37 °C for 4 h, and short sonication handling times were required to attain nano-scaled liposomes. Under conditions of high temperature, acidity and alkalinity, lysozyme nanoliposomes are unstable. View Full-Text
Keywords: lysozyme; nanoliposomes; response surface methodology; optimization; stability lysozyme; nanoliposomes; response surface methodology; optimization; stability
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wu, Z.; Guan, R.; Lyu, F.; Liu, M.; Gao, J.; Cao, G. Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability. Molecules 2016, 21, 741.

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