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Molecules 2016, 21(6), 697; doi:10.3390/molecules21060697

Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

1
School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China
2
School of Food Science and Technology, National Engineering Research Center of Seafood, National and Local Joint Engineering Laboratory for Marine Bioactive Polysaccharide Development and Application, Dalian Polytechnic University, Dalian 116034, China
3
Clinical Laboratory, Dalian Municipal Central Hospital Affiliated of Dalian Medical University, Dalian 116033, China
*
Author to whom correspondence should be addressed.
Academic Editors: Isabel C. F. R. Ferreira and Nancy D. Turner
Received: 4 April 2016 / Revised: 20 May 2016 / Accepted: 23 May 2016 / Published: 8 June 2016
(This article belongs to the Collection Bioactive Compounds)
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Abstract

In this study, we aimed at characterizing the structure and the anticoagulant activity of a polysaccharide fraction (AGP33) isolated from the gonads of Haliotis discus hannai Ino. AGP33 was extracted by enzymatic hydrolysis and purified by ion-exchange and gel-filtration chromatography. The backbone fraction of AGP33 (BAGP33), which appeared to contain of mannose, glucose and galactose, was prepared by partial acid hydrolysis. According to methylation and nuclear magnetic resonance (NMR) spectroscopy, the backbone of AGP33 was identified as mainly consisting of 1→3-linked, 1→4-linked, and 1→6-linked monosaccharides. AGP33 is a sulfated polysaccharide with sulfates occur at 3-O- and 4-O-positions. It prolonged thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) compared to a saline control solution in a dosage-dependent manner. AGP33 exhibited an extension (p < 0.01) of APTT compared to the saline group at concentrations higher than 5 μg/mL. AGP33 exhibited higher anticoagulant activity than its desulfated product (AGP33-des) and BAGP33. The results showed that polysaccharide with higher molecular weight and sulfate content demonstrated greater anticoagulant activity. View Full-Text
Keywords: polysaccharide; Haliotis discus hannai; APTT; TT; PT; backbone polysaccharide; Haliotis discus hannai; APTT; TT; PT; backbone
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhao, J.; Yang, J.; Song, S.; Zhou, D.; Qiao, W.; Zhu, C.; Liu, S.; Zhu, B. Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad. Molecules 2016, 21, 697.

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