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Molecules 2016, 21(6), 747; doi:10.3390/molecules21060747

Development and Validation of an Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneous Determination of Four Type B Trichothecenes and Masked Deoxynivalenol in Various Feed Products

1,2,†
,
1,†
,
1
,
1
,
1
,
1
and
1,*
1
Institute for Agro-food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
2
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 9 March 2016 / Revised: 21 May 2016 / Accepted: 30 May 2016 / Published: 8 June 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1730 KB, uploaded 8 June 2016]   |  

Abstract

A reliable and sensitive analytical method was developed for simultaneous determination of deoxynivalenol(DON), 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON), fusarenon X (FUS-X), and masked deoxynivalenol (deoxynivalenol-3-glucoside, D3G) in formula feed, concentrated feed, and premixed feed products. The method was based on an improved sample pretreatment with the commercially available HLB cartridges used for sample purification and enrichment followed by analysis using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Several key parameters including the extraction solvents, the positions of sample loading solvents, washing and elution solvents for HLB cartridges were carefully optimized to achieve optimal extraction and purification efficiencies. The established method was extensively validated by determining the linearity (R2 ≥ 0.99), sensitivity (limit of quantification in the range of 0.08–4.85 μg/kg), recovery (79.3%–108.1%), precision (Intra-day RSDs ≤ 13.5% and Inter-day RSDs ≤ 14.9%), and then was successfully applied to determine the four type B trichothecenes and D3G in a total of 31 feed samples. Among them, 26 were contaminated with various mycotoxins at the levels of 2.1–864.5 μg/kg, and D3G has also been detected in 17 samples with the concentrations in the range of 2.1–34.8 μg/kg, proving the established method to be a valuable tool for type B trichothecenes and masked DON monitoring in complex feed matrices. View Full-Text
Keywords: type B trichothecenes; masked deoxynivalenol; HLB cartridges; feed products; ultra-high performance liquid chromatography-tandem mass spectrometry type B trichothecenes; masked deoxynivalenol; HLB cartridges; feed products; ultra-high performance liquid chromatography-tandem mass spectrometry
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Fan, Z.; Bai, B.; Jin, P.; Fan, K.; Guo, W.; Zhao, Z.; Han, Z. Development and Validation of an Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Simultaneous Determination of Four Type B Trichothecenes and Masked Deoxynivalenol in Various Feed Products. Molecules 2016, 21, 747.

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