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Molecules 2016, 21(5), 640; doi:10.3390/molecules21050640

Simultaneous Qualitative and Quantitative Analysis of Multiple Chemical Constituents in YiQiFuMai Injection by Ultra-Fast Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry

1,†
,
2,†
,
2
,
2
,
1
,
1,3,* and 1,*
1
Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China
2
Department of Technology Development, TianJin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300410, China
3
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Thomas J. Schmidt
Received: 26 February 2016 / Revised: 9 May 2016 / Accepted: 11 May 2016 / Published: 18 May 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [3518 KB, uploaded 18 May 2016]   |  

Abstract

YiQiFuMai injection (YQFM) is a modern lyophilized powder preparation derived from the traditional Chinese medicine Sheng-mai san (SMS) used for treating cardiovascular diseases, such as chronic heart failure. However, its chemical composition has not been fully elucidated, particularly for the preparation derived from Ophiopogon japonicus. This study aimed to establish a systematic and reliable method to quickly and simultaneously analyze the chemical constituents in YQFM by ultra-fast liquid chromatography coupled with ion trap time-of-flight mass spectrometry (UFLC-IT-TOF/MS). Sixty-five compounds in YQFM were tentatively identified by comparison with reference substances or literature data. Furthermore, twenty-one compounds, including three ophiopogonins, fifteen ginsenosides and three lignans were quantified by UFLC-IT-TOF/MS. Notably, this is the first determination of steroidal saponins from O. japonicus in YQFM. The relative standard deviations (RSDs) of intra- and inter-day precision, reproducibility and stability were <4.9% and all analytes showed good linearity (R2 ≥ 0.9952) and acceptable recovery of 91.8%–104.2% (RSD ≤ 5.4%), indicating that the methods were reliable. These methods were successfully applied to quantitative analysis of ten batches of YQFM. The developed approach can provide useful and comprehensive information for quality control, further mechanistic studies in vivo and clinical application of YQFM. View Full-Text
Keywords: YiQiFuMai injection; UFLC-IT-TOF/MS; ophiopogonins; identification; quantification YiQiFuMai injection; UFLC-IT-TOF/MS; ophiopogonins; identification; quantification
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MDPI and ACS Style

Liu, C.; Ju, A.; Zhou, D.; Li, D.; Kou, J.; Yu, B.; Qi, J. Simultaneous Qualitative and Quantitative Analysis of Multiple Chemical Constituents in YiQiFuMai Injection by Ultra-Fast Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry. Molecules 2016, 21, 640.

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