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Molecules 2016, 21(5), 592; doi:10.3390/molecules21050592

Developing an Absorption–Based Quality Control Method for Hu–Gan–Kang–Yuan Capsules by UFLC–QTOF–MS/MS Screening and HPLC–DAD Quantitative Determination

College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China
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Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 8 April 2016 / Revised: 27 April 2016 / Accepted: 28 April 2016 / Published: 18 May 2016
(This article belongs to the Section Natural Products)
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Abstract

Traditional Chinese Medicine Preparations (TCMPs) contain massive numbers of ingredients responsible for their multiple efficacies. An absorption–based quality control method for complicated TCMPs using Hu–gan–kang–yuan Capsule (HGKYC) as an example was developed. To select proper chemical markers for quality control of HGKYC, an ultra–fast liquid chromatography (UFLC) coupled with electrospray ionization quadrupole time–off light mass spectrometry (UFLC–QTOF–MS/MS) method was used for the rapid separation and structural identification of the constituents in the HGKYC extract and the rat serum after oral administration of HGKYC. As a result, one hundred and seven prototype constituents including flavonoids, organic acid, phenylpropanoids, anthraquinones, saponins, alkaloids, terpenes, phenols and amino acids in HGKYC extract, and 43 compounds found in rat serum after oral administration of HGKYC were unambiguously identified or tentatively characterized by comparing retention times and MS information with those of authentic standards or available literature references. Finally, a simple, low–cost and effective method of simultaneous determination for baicalein, wogonin, paeonol and emodin in HGKYC was developed using high performance liquid chromatography coupled with a diode array detector. In conclusion, an absorption–based quality control pattern was developed and successfully used for evaluating HGKYC. View Full-Text
Keywords: hu–gan–kang–yuan capsule; quality control; UFLC–QTOF–MS/MS; absorption hu–gan–kang–yuan capsule; quality control; UFLC–QTOF–MS/MS; absorption
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Wei, F.; Chen, M.; Luo, C.; Chen, F.; Shen, Q.; Mo, Z. Developing an Absorption–Based Quality Control Method for Hu–Gan–Kang–Yuan Capsules by UFLC–QTOF–MS/MS Screening and HPLC–DAD Quantitative Determination. Molecules 2016, 21, 592.

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