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Molecules 2016, 21(5), 618; doi:10.3390/molecules21050618

Preparation of Pd/Bacterial Cellulose Hybrid Nanofibers for Dopamine Detection

Key Laboratory of Eco–Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, Jiang Su, China
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Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 24 March 2016 / Revised: 27 April 2016 / Accepted: 4 May 2016 / Published: 11 May 2016
(This article belongs to the Section Bioorganic Chemistry)
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Abstract

Palladium nanoparticle-bacterial cellulose (PdBC) hybrid nanofibers were synthesized by in-situ chemical reduction method. The obtained PdBC nanofibers were characterized by a series of analytical techniques. The results revealed that Pd nanoparticles were evenly dispersed on the surfaces of BC nanofibers. Then, the as-prepared PdBC nanofibers were mixed with laccase (Lac) and Nafion to obtain mixture suspension, which was further modified on electrode surface to construct novel biosensing platform. Finally, the prepared electrochemical biosensor was employed to detect dopamine. The analysis result was satisfactory, the sensor showed excellent electrocatalysis towards dopamine with high sensitivity (38.4 µA·mM−1), low detection limit (1.26 µM), and wide linear range (5–167 µM). Moreover, the biosensor also showed good repeatability, reproducibility, selectivity and stability and was successfully used in the detection of dopamine in human urine, thus providing a promising method for dopamine analysis in clinical application. View Full-Text
Keywords: palladium nanoparticle; bacterial cellulose; laccase; dopamine; biosensor palladium nanoparticle; bacterial cellulose; laccase; dopamine; biosensor
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Li, D.; Ao, K.; Wang, Q.; Lv, P.; Wei, Q. Preparation of Pd/Bacterial Cellulose Hybrid Nanofibers for Dopamine Detection. Molecules 2016, 21, 618.

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