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Molecules 2016, 21(4), 408; doi:10.3390/molecules21040408

Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry

1
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
College of Ocean and Environment, Xiamen University, Xiamen 361005, China
3
Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 11 January 2016 / Revised: 21 March 2016 / Accepted: 23 March 2016 / Published: 25 March 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [2425 KB, uploaded 25 March 2016]   |  

Abstract

To evaluate the nutritional and functional value of Sipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitative determination of 25 free amino acids and 16 nucleosides and nucleobases in S. nudus within 20 min, which was confirmed to be reproducible and accurate. The limits of detection (LODs) and quantification (LOQs) were between 0.003–0.229 μg/mL and 0.008–0.763 μg/mL for the 41 analytes, respectively. The established method was applied to analyze 19 batches of S. nudus samples from four habitats with two different processing methods. The results showed that S. nudus contained a variety of free amino acids, nucleosides and nucleobases in sufficient quantity and reasonable proportion. They also demonstrated that the contents of these compounds in different parts of S. nudus were significantly discriminating, which were in the order: (highest) coelomic fluid > body wall > intestine (lowest). The method is simple and accurate, and could serve as a technical support for establishing quality control of S. nudus and other functional seafoods. Moreover, the research results also laid foundation for further exploitation and development of S. nudus. View Full-Text
Keywords: UPLC-TQ-MS/MS; Sipunculus nudus; free amino acid; nucleoside; nucleobase UPLC-TQ-MS/MS; Sipunculus nudus; free amino acid; nucleoside; nucleobase
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Ge, Y.; Tang, Y.; Guo, S.; Liu, X.; Zhu, Z.; Zhang, L.; Liu, P.; Ding, S.; Lin, X.; Lin, R.; Duan, J.-A. Simultaneous Quantitation of Free Amino Acids, Nucleosides and Nucleobases in Sipunculus nudus by Ultra-High Performance Liquid Chromatography with Triple Quadrupole Mass Spectrometry. Molecules 2016, 21, 408.

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