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Molecules 2016, 21(4), 397; doi:10.3390/molecules21040397

Extraction Optimization, Purification and Physicochemical Properties of Polysaccharides from Gynura medica

1
College of Prataculture Science, Nanjing Agricultural University, Nanjing 210095, China
2
School of Marine and Bioengineering, Yan Cheng Institute of Technology, Yan Cheng 224051, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 1 March 2016 / Revised: 18 March 2016 / Accepted: 18 March 2016 / Published: 23 March 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [2813 KB, uploaded 23 March 2016]   |  

Abstract

Extraction of polysaccharides from Gynura medica (GMPs) was optimized by response surface methodology (RSM). A central composition design including three parameters, namely extraction temperature (X1), ratio of water to raw material (X2) and extraction time (X3), was used. The best conditions were extraction temperature of 91.7 °C, extraction time of 4.06 h and ratio of water to raw material of 29.1 mL/g. Under the optimized conditions, the yield of GMPs was 5.56%, which was similar to the predicted polysaccharides yield of 5.66%. A fraction named GMP-1 was obtained after isolation and purification by DEAE-52 and Sephadex G-100 gel chromatography, respectively. GMP-1, with a molecular weight of 401 kDa, mainly consisted of galacturonic acid (GalA), xylose (Xyl), glucose (Glu). Infrared spectroscopy was used to characterize the major functional groups of GMP-1 and the results indicated that it was an acidic polysaccharide. The antioxidant and α-glucosidase inhibitory activities of GMPs and GMP-1 were determined in vitro. The results indicated that GMPs and GMP-1 show potential for use in functional foods or medicines. View Full-Text
Keywords: Gynura medica; extraction optimization; purification; polysaccharides; activity Gynura medica; extraction optimization; purification; polysaccharides; activity
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Li, F.; Gao, J.; Xue, F.; Yu, X.; Shao, T. Extraction Optimization, Purification and Physicochemical Properties of Polysaccharides from Gynura medica. Molecules 2016, 21, 397.

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