Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice
AbstractIn this study, we found that chloroform fraction (CF) from TJP ethanolic extract inhibited lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and intracellular ROS in RAW264.7 cells. In addition, expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) genes was reduced, as evidenced by western blot. Our results indicate that CF exerts anti-inflammatory effects by down-regulating expression of iNOS and COX-2 genes through inhibition of MAPK (ERK, JNK and p38) and NF-κB signaling. Similarly we also evaluated the effects of CF on LPS-induced acute lung injury. Male Balb/c mice were pretreated with dexamethasone or CF 1 h before intranasal instillation of LPS. Eight hours after LPS administration, the inflammatory cells in the bronchoalveolar lavage fluid (BALF) were determined. The results indicated that CF inhibited LPS-induced TNF-α and IL-6 production in a dose dependent manner. It was also observed that CF attenuated LPS-induced lung histopathologic changes. In conclusion, these data demonstrate that the protective effect of CF on LPS-induced acute lung injury (ALI) in mice might relate to the suppression of excessive inflammatory responses in lung tissue. Thus, it can be suggested that CF might be a potential therapeutic agent for ALI. View Full-Text
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Kim, Y.-S.; Hwang, J.-W.; Jang, J.-H.; Son, S.; Seo, I.-B.; Jeong, J.-H.; Kim, E.-H.; Moon, S.-H.; Jeon, B.-T.; Park, P.-J. Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice. Molecules 2016, 21, 392.
Kim Y-S, Hwang J-W, Jang J-H, Son S, Seo I-B, Jeong J-H, Kim E-H, Moon S-H, Jeon B-T, Park P-J. Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice. Molecules. 2016; 21(3):392.Chicago/Turabian Style
Kim, Yon-Suk; Hwang, Jin-Woo; Jang, Jae-Hyuk; Son, Sangkeun; Seo, Il-Bok; Jeong, Jae-Hyun; Kim, Ee-Hwa; Moon, Sang-Ho; Jeon, Byong-Tae; Park, Pyo-Jam. 2016. "Trapa japonica Pericarp Extract Reduces LPS-Induced Inflammation in Macrophages and Acute Lung Injury in Mice." Molecules 21, no. 3: 392.
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