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Molecules 2016, 21(3), 315; doi:10.3390/molecules21030315

Isolation, Purification and Quantification of Ginsenoside F5 and F3 Isomeric Compounds from Crude Extracts of Flower Buds of Panax ginseng

College of Medical, Dalian University, Dalian 116622, China
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Academic Editor: Christopher W. K. Lam
Received: 26 December 2015 / Revised: 24 February 2016 / Accepted: 3 March 2016 / Published: 9 March 2016
(This article belongs to the Collection Herbal Medicine Research)
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Abstract

In this paper, the isolation, purification and quantification of ginsenoside F5 and F3 isomeric compounds from crude extracts of flower buds of Panax ginseng (CEFBPG) was investigated by reversed-phase high-performance liquid chromatography (RP-HPLC) for the first time. The satisfied separation at analytical scale was achieved using a Zorbax Eclipse XDB C-18 column with a ternary mobile phase of acetonitrile–water–phosphoric acid (28:71:1) at a flow rate of 1.0 mL/min within 40 min. UV detection was set at 203 nm. Ginsenoside F5 and F3 was 4.21 mg and 5.13 mg in 1 g flower buds of P. ginseng (FBPG), respectively. The preparation of ginsenoside F5 and F3 at semi-preparative scale was performed by using a Daisogel C-18 column and gradient elution system of acetonitrile–water (32:68 → 28:72) at a flow rate of 10 mL/min with a sample load of 20–30 mg, and yielded ginsenosides in purity of more than 96%. Their structures were characterized by NMR and high resolution electrospray ionization mass spectrometry (HRESIMS). All the method validations showed acceptable limits. The results indicate a new source to obtain ginsenoside F5 and F3, and show that the method developed here appears to be reliable for simultaneously preparing them from CEFBPG. View Full-Text
Keywords: ginsenoside F5; ginsenoside F3; isomer; purification; quantification ginsenoside F5; ginsenoside F3; isomer; purification; quantification
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Li, K.-K.; Xu, F.; Gong, X.-J. Isolation, Purification and Quantification of Ginsenoside F5 and F3 Isomeric Compounds from Crude Extracts of Flower Buds of Panax ginseng. Molecules 2016, 21, 315.

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