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Molecules 2016, 21(3), 321; doi:10.3390/molecules21030321

Liquid Chromatography with Tandem Mass Spectrometry: A Sensitive Method for the Determination of Dehydrodiisoeugenol in Rat Cerebral Nuclei

1
State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking University, No. 38, Xueyuan Road, Haidian District, Beijing 100191, China
2
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Isabel C. F. R. Ferreira
Received: 24 January 2016 / Revised: 26 February 2016 / Accepted: 1 March 2016 / Published: 9 March 2016
(This article belongs to the Collection Bioactive Compounds)
View Full-Text   |   Download PDF [741 KB, uploaded 9 March 2016]   |  

Abstract

A new liquid chromatography–tandem mass spectrometry (LC-MS/MS) method is developed for the quantification of dehydrodiisoeugenol (DDIE) in rat cerebral nuclei after single intravenous administration. DDIE and daidzein (internal standard) were separated on a Diamonsil™ ODS C18 column with methanol–water containing 0.1% formic acid (81:19, v/v) as a mobile phase. Detection of DDIE was performed on a positive electrospray ionization source using a triple quadrupole mass spectrometer. DDIE and daidzein were monitored at m/z 327.2→188.0 and m/z 255.0→199.2, respectively, in multiple reaction monitoring mode. This method enabled quantification of DDIE in various brain areas, including, cortex, hippocampus, striatum, hypothalamus, cerebellum and brainstem, with high specificity, precision, accuracy, and recovery. The data herein demonstrate that our new LC-MS/MS method is highly sensitive and suitable for monitoring cerebral nuclei distribution of DDIE. View Full-Text
Keywords: HPLC-MS/MS; cerebral nuclei; drug distribution; nutmeg; Myristica fragrans; dehydrodiisoeugenol HPLC-MS/MS; cerebral nuclei; drug distribution; nutmeg; Myristica fragrans; dehydrodiisoeugenol
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Zhang, Y.-B.; Yang, X.-B.; Yang, X.-W.; Xu, W.; Li, F.; Gonzezal, F.J. Liquid Chromatography with Tandem Mass Spectrometry: A Sensitive Method for the Determination of Dehydrodiisoeugenol in Rat Cerebral Nuclei. Molecules 2016, 21, 321.

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