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Molecules 2016, 21(2), 139; doi:10.3390/molecules21020139

Antibacterial and Synergistic Activity of Pentacyclic Triterpenoids Isolated from Alstonia scholaris

1
Research Center for Biodiversity, China Medical University, Taichung, 40402, Taiwan
2
Department of Veterinary Medicine, College of Veterinary Medicine, National Chung-Hsing University, Taichung, 40402, Taiwan
*
Author to whom correspondence should be addressed.
Academic Editor: Christopher W. K. Lam
Received: 24 December 2015 / Revised: 12 January 2016 / Accepted: 21 January 2016 / Published: 25 January 2016
(This article belongs to the Collection Herbal Medicine Research)
View Full-Text   |   Download PDF [662 KB, uploaded 25 January 2016]   |  

Abstract

(1) Background: Alstonia scholaris (Apocynaceae) is an important medicinal plant that has been historically used in “Dai” ethnopharmacy to treat infectious diseases in China. Although various pharmacological activities have been reported, the antimicrobial constitutes of A. scholaris have not yet been identified. The objective of this study is to evaluate the antibacterial constitutes from the leaf extract of A. scholaris and to assess the synergistic effects of isolated compounds with antibiotics against bacterial pathogens.; (2) Methods: The chemical constitutes isolated from the leaf extract of A. scholaris were structurally identified by NMR. The antibacterial and synergistic effect of compounds was assessed by calculating the minimal inhibitory concentration (MIC), checkerboard dilution test, and time-kill assay.; (3) Results: Six pentacyclic triterpenoids were structurally identified as (1) lupeol, (2) betulin, (3) 3-hydroxy-11-ursen-28,13-olide, (4) betulinic acid, (5) oleanolic acid and (6) ursolic acid. Both oleanolic and ursolic acid showed antibacterial activity but were limited to Gram-positive bacteria. Ursolic acid showed a synergistic effect with ampicillin and tetracycline against both Bacillus cereus and S. aureus.; (4) Conclusion: These findings reflect that pentacyclic triterpenoids are the antibacterial chemicals in A. scholaris. The ability of ursolic acid to enhance the activity of antibiotics can constitute a valuable group of therapeutic agents in the future. View Full-Text
Keywords: Alstonia scholaris; triterpenoid; antibacterial; synergistic; ursolic acid; MRSA Alstonia scholaris; triterpenoid; antibacterial; synergistic; ursolic acid; MRSA
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Wang, C.-M.; Chen, H.-T.; Wu, Z.-Y.; Jhan, Y.-L.; Shyu, C.-L.; Chou, C.-H. Antibacterial and Synergistic Activity of Pentacyclic Triterpenoids Isolated from Alstonia scholaris. Molecules 2016, 21, 139.

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