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Molecules 2016, 21(12), 1662; doi:10.3390/molecules21121662

Activity of Eribulin in a Primary Culture of Well-Differentiated/Dedifferentiated Adipocytic Sarcoma

1
Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Via Piero Maroncelli 40, 47014 Meldola (FC), Italy
2
Pathology Unit, Morgagni-Pierantoni Hospital, Via Carlo Forlanini 34, 47121 Forlì, Italy
3
Unit of Surgery and Advanced Oncologic Therapies, Morgagni-Pierantoni Hospital, Via Carlo Forlanini 34, 47121 Forlì, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Wei Li
Received: 30 August 2016 / Revised: 16 November 2016 / Accepted: 29 November 2016 / Published: 3 December 2016
(This article belongs to the Special Issue Tubulin Inhibitors)
View Full-Text   |   Download PDF [2617 KB, uploaded 3 December 2016]   |  

Abstract

Eribulin mesylate is a novel, non-taxane, synthetic microtubule inhibitor showing antitumor activity in a wide range of tumors including soft tissue sarcomas (STS). Eribulin has been recently approved for the treatment of metastatic liposarcoma (LPS) patients previously treated with anthracyclines. This work investigated the mechanism of action of this innovative antitubulin agent in well-differentiated/dedifferentiated LPS (ALT/DDLPS) which represents one of the most common adipocytic sarcoma histotypes. A primary culture of ALT/DDLPS from a 54-year-old patient was established. The anticancer activity of eribulin on the patient-derived primary culture was assessed by MTT and tunel assays. Eribulin efficacy was compared to other drugs approved for the treatment of STS. Cell migration and morphology were examined after exposure to eribulin to better understand the drug mechanism of action. Finally, Western blot analysis of apoptosis and migration proteins was performed. The results showed that eribulin exerts its antiproliferative effect by the arrest of cell motility and induction of apoptosis. Our results highlighted the activity of eribulin in the treatment of ALT/DDLPS patients. View Full-Text
Keywords: eribulin; microtubule inhibitors; adipocytic sarcoma; primary culture eribulin; microtubule inhibitors; adipocytic sarcoma; primary culture
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De Vita, A.; Miserocchi, G.; Recine, F.; Mercatali, L.; Pieri, F.; Medri, L.; Bongiovanni, A.; Cavaliere, D.; Liverani, C.; Spadazzi, C.; Amadori, D.; Ibrahim, T. Activity of Eribulin in a Primary Culture of Well-Differentiated/Dedifferentiated Adipocytic Sarcoma. Molecules 2016, 21, 1662.

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