Next Article in Journal
cDNA Isolation and Functional Characterization of UDP-d-glucuronic Acid 4-Epimerase Family from Ornithogalum caudatum
Next Article in Special Issue
Ultrasound-Assisted Extraction May Not Be a Better Alternative Approach than Conventional Boiling for Extracting Polysaccharides from Herbal Medicines
Previous Article in Journal
Flavonoids-Rich Orthosiphon stamineus Extract as New Candidate for Angiotensin I-Converting Enzyme Inhibition: A Molecular Docking Study
Previous Article in Special Issue
Tea Polysaccharides and Their Bioactivities
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(11), 1496; doi:10.3390/molecules21111496

Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose

1
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Wangjing Zhonghuan Road No. 6 School Range, Chaoyang District, Beijing 100102, China
2
Beijing Chinese Medicine Research Institute, Beijing University of Chinese Medicine, North Third Ring Road No. 11 School Range, Chaoyang District, Beijing 100029, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Quan-Bin Han
Received: 26 September 2016 / Revised: 2 November 2016 / Accepted: 2 November 2016 / Published: 9 November 2016
(This article belongs to the Special Issue Natural Polysaccharides)
View Full-Text   |   Download PDF [5733 KB, uploaded 9 November 2016]   |  

Abstract

Fuzi has been used to treat diabetic complications for many years in china. In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury. Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated. Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn). The results witnessed the efficiency of this method and seven monosaccharides were tentatively identified, among which fucose was first reported. Simultaneously, m/z 215 can be considered as diagnostic ions to confirm the number of monosaccharides. Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group. After treatment for 48 h, the data showed FPS could significantly decrease the intracellular ROS and apoptosis, which were determined by the corresponding fluorescent probes. Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot. Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level. Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly. Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage. View Full-Text
Keywords: Fuzi polysaccharides; HPLC-ESI-MSn; monosaccharide composition; Schwann cells; antioxidant; AMPK-PGC-1α pathway Fuzi polysaccharides; HPLC-ESI-MSn; monosaccharide composition; Schwann cells; antioxidant; AMPK-PGC-1α pathway
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, B.-B.; Wang, J.-L.; Yuan, J.; Quan, Q.-H.; Ji, R.-F.; Tan, P.; Han, J.; Liu, Y.-G. Sugar Composition Analysis of Fuzi Polysaccharides by HPLC-MSn and Their Protective Effects on Schwann Cells Exposed to High Glucose. Molecules 2016, 21, 1496.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top