Next Article in Journal
Statistical Significance of the Maximum Hardness Principle Applied to Some Selected Chemical Reactions
Next Article in Special Issue
Organ- and Growing Stage-Specific Expression of Solanesol Biosynthesis Genes in Nicotiana tabacum Reveals Their Association with Solanesol Content
Previous Article in Journal
Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS
Previous Article in Special Issue
Cytotoxic, Antitumor and Immunomodulatory Effects of the Water-Soluble Polysaccharides from Lotus (Nelumbo nucifera Gaertn.) Seeds
Article Menu
Issue 11 (November) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(11), 1479; doi:10.3390/molecules21111479

Medium Optimization and Fermentation Kinetics for κ-Carrageenase Production by Thalassospira sp. Fjfst-332

1
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, China
2
Fujian Provincial Technical Research Center of Marine Food and Biological Products Processing, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, China
*
Author to whom correspondence should be addressed.
Academic Editors: Pinarosa Avato and Jianbo Xiao
Received: 11 August 2016 / Revised: 17 October 2016 / Accepted: 31 October 2016 / Published: 5 November 2016
View Full-Text   |   Download PDF [8440 KB, uploaded 5 November 2016]   |  

Abstract

Effective degradation of κ-carrageenan by isolated Thalassospira sp. fjfst-332 is reported for the first time in this paper. It was identified by 16S rDNA sequencing and morphological observation using Transmission Electron Microscopy (TEM). Based on a Plackett–Burman design for significant variables, Box–Behnken experimental design and response surface methodology were used to optimize the culture conditions. Through statistical optimization, the optimum medium components were determined as follows: 2.0 g/L κ-carrageenan, 1.0 g/L yeast extract, 1.0 g/L FOS, 20.0 g/L NaCl, 2.0 g/L NaNO3, 0.5 g/L MgSO4·7H2O, 0.1 g/L K2HPO4, and 0.1 g/L CaCl2. The highest activity exhibited by Thalassospira sp. fjfst-332 was 267 U/mL, which makes it the most vigorous wild bacterium for κ-carrageenan production. In order to guide scaled-up production, two empirical models—the logistic equation and Luedeking–Piretequation—were proposed to predict the strain growth and enzyme production, respectively. Furthermore, we report the fermentation kinetics and every empirical equation of the coefficients (α, β, X0, Xm and μm) for the two models, which could be used to design and optimize industrial processes. View Full-Text
Keywords: κ-carrageenase; Thalassospira sp.; response surface methodology; kinetics model κ-carrageenase; Thalassospira sp.; response surface methodology; kinetics model
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Guo, J.; Zhang, L.; Lu, X.; Zeng, S.; Zhang, Y.; Xu, H.; Zheng, B. Medium Optimization and Fermentation Kinetics for κ-Carrageenase Production by Thalassospira sp. Fjfst-332. Molecules 2016, 21, 1479.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top