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Molecules 2016, 21(11), 1471; doi:10.3390/molecules21111471

Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS

1
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China
2
State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, China
3
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 27 September 2016 / Revised: 31 October 2016 / Accepted: 1 November 2016 / Published: 5 November 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [9323 KB, uploaded 5 November 2016]   |  

Abstract

Radix astragali is widely used either as a single herb or as a collection of herbs in a complex prescription in China. In this study, bovine serum albumin functionalized magnetic nanoparticles (BSA-MN) coupled with high performance liquid chromatography-mass spectrometry (HPLC-MS) were used to screen and identify bound ligands from the n-butanol part of a Radix astragali extract. The prepared BSA-MN showed sufficient magnetic response for the separation with an ordinary magnet and satisfied reusability. Fundamental parameters affecting the preparation of BSA-MN and the screening efficiency were studied and optimized. Under the optimum conditions, four bound ligands were screened out from the n-butanol part of a Radix astragali extract and identified as genistin (1), calycosin-7-O-β-d-glucoside (2), ononin (3) and formononetin (4). This effective method could be widely applied for rapid screening and identification of active compounds from complex mixtures without the need for preparative isolation. View Full-Text
Keywords: bovine serum albumin; HPLC-MS; ligands; magnetic nanoparticles; Radix astragali bovine serum albumin; HPLC-MS; ligands; magnetic nanoparticles; Radix astragali
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Liu, L.; Leng, J.; Yang, X.; Liao, L.; Cen, Y.; Xiao, A.; Ma, L. Rapid Screening and Identification of BSA Bound Ligands from Radix astragali Using BSA Immobilized Magnetic Nanoparticles Coupled with HPLC-MS. Molecules 2016, 21, 1471.

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