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Molecules 2016, 21(10), 1280; doi:10.3390/molecules21101280

Cytoproliferative and Cytoprotective Effects of Striatisporolide A Isolated from Rhizomes of Athyrium multidentatum (Doell.) Ching on Human Umbilical Vein Endothelial Cells

1
Department of Pharmacy, Weifang Medical University, Weifang 261053, China
2
Analysis and Test Center, Yianbian University, Yanji 133002, China
3
Plastic Surgery Institute of Weifang Medical University, Weifang 261053, China
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 21 August 2016 / Revised: 18 September 2016 / Accepted: 20 September 2016 / Published: 24 September 2016
(This article belongs to the Section Natural Products)
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Abstract

Objectives: The aim of this study was to investigate the proliferative and protective effects of striatisporolide A (SA) obtained from the rhizomes of Athyrium multidentatum (Doell.) Ching on human umbilical vein endothelial cells (HUVECs). Methods: Cell viability was measured by the MTT method. Cell apoptosis was determined by flow cytometry. Intracellular ROS was measured by the 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe. Results: The viability rate in cells treated with 100 µM SA alone was increased to 128.72% ± 0.19% and showed a significant difference compared with the control group (p < 0.05). Meanwhile, SA augmented the cell viabilities in H2O2-treated HUVECs, and the cell viability was enhanced to 56.94% ± 0.13% (p < 0.01) when pre-incubated with 50 µM SA. The cell apoptosis rates were reduced to 2.17% ± 0.20% (p < 0.05) and 3.1% ± 0.34% (p < 0.01), respectively, after treatment with SA alone or SA/H2O2. SA inhibited the overproduction of reactive oxygen species (ROS) in HUVECs induced by H2O2 and the fluorescent intensity was abated to 9.47 ± 0.61 after pre-incubated with 100 μM SA. Conclusions: The biological activities of SA were explored for the first time. Our results stated that SA exhibited significant cytoproliferative and minor cytoprotective effects on HUVECs. We presume that the mechanisms of the proliferation and protection actions of SA involve interference with the generation of ROS and the cell apoptosis. These findings provide a new perspective on the biological potential of butenolides. View Full-Text
Keywords: striatisporolide A; Athyrium multidentatum (Doell.) Ching; butenolides; cytoprotective effect; ROS; apoptosis striatisporolide A; Athyrium multidentatum (Doell.) Ching; butenolides; cytoprotective effect; ROS; apoptosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, D.-M.; Sheng, J.-W.; Wang, S.-H.; Zhang, W.-F.; Zhang, W.; Zhang, D.-J. Cytoproliferative and Cytoprotective Effects of Striatisporolide A Isolated from Rhizomes of Athyrium multidentatum (Doell.) Ching on Human Umbilical Vein Endothelial Cells. Molecules 2016, 21, 1280.

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