Next Article in Journal
Evidence for the Formation of Benzacridine Derivatives in Alkaline-Treated Sunflower Meal and Model Solutions
Previous Article in Journal
Synthesis of Isotopically Labeled 13C3-Simazine and Development of a Simultaneous UPLC-MS/MS Method for the Analysis of Simazine in Soil
Previous Article in Special Issue
Silymarin Constituents Enhance ABCA1 Expression in THP-1 Macrophages
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(1), 90; doi:10.3390/molecules21010090

The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr

Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a, Lublin 20-093, Poland
Orthopaedics and Rehabilitation Clinic, Medical University Lublin, Chodźki 4a, Lublin 20-093, Poland
Department of Plant Physiology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Akademicka 19, Lublin 20-033, Poland
Botanical Garden of Maria Curie-Skłodowska University in Lublin, Sławinkowska 3, Lublin 20-810, Poland
Department of Biopharmacy, Medical University of Lublin, Chodźki 4a, Lublin 20-093, Poland
Department of Inorganic Chemistry, Medical University of Lublin, Chodźki 4a, Lublin 20-093, Poland
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 4 November 2015 / Revised: 22 December 2015 / Accepted: 7 January 2016 / Published: 14 January 2016
View Full-Text   |   Download PDF [643 KB, uploaded 14 January 2016]   |  


The amount of secondary metabolites in plants can be enhanced or reduced by various external factors. In this study, the effect of strontium ions on the production of phytoestrogens in soybeans was investigated. The plants were treated with Hoagland’s solution, modified with Sr2+ with concentrations ranging from 0.5 to 3.0 mM, and were grown for 14 days in hydroponic cultivation. After harvest, soybean plants were separated into roots and shoots, dried, and pulverized. The plant material was extracted with methanol and hydrolyzed. Phytoestrogens were quantified by HPLC. The significant increase in the concentration of the compounds of interest was observed for all tested concentrations of strontium ions when compared to control. Sr2+ at a concentration of 2 mM was the strongest elicitor, and the amount of phytoestrogens in plant increased ca. 2.70, 1.92, 3.77 and 2.88-fold, for daidzein, coumestrol, genistein and formononetin, respectively. Moreover, no cytotoxic effects were observed in HepG2 liver cell models after treatment with extracts from 2 mM Sr2+-stressed soybean plants when compared to extracts from non-stressed plants. Our results indicate that the addition of strontium ions to the culture media may be used to functionalize soybean plants with enhanced phytoestrogen content. View Full-Text
Keywords: soy; Glycine max; phytoestrogens; isoflavones; coumestrol; strontium soy; Glycine max; phytoestrogens; isoflavones; coumestrol; strontium

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wójciak-Kosior, M.; Sowa, I.; Blicharski, T.; Strzemski, M.; Dresler, S.; Szymczak, G.; Wnorowski, A.; Kocjan, R.; Świeboda, R. The Stimulatory Effect of Strontium Ions on Phytoestrogens Content in Glycine max (L.) Merr. Molecules 2016, 21, 90.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top