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Molecules 2016, 21(1), 84; doi:10.3390/molecules21010084

Quantitative Analysis of Differential Proteome Expression in Epithelial-to-Mesenchymal Transition of Bladder Epithelial Cells Using SILAC Method

1
The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China
2
Wuxi Medical School, Jiangnan University, Wuxi 214122, China
3
Department of Urology, Affiliated Hospital of Jiangnan University, Wuxi 214062, China
4
Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an 710069, China
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 12 November 2015 / Revised: 7 January 2016 / Accepted: 8 January 2016 / Published: 15 January 2016
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [1263 KB, uploaded 15 January 2016]   |  

Abstract

Epithelial-to-mesenchymal transition (EMT) is an essential biological process involved in embryonic development, cancer progression, and metastatic diseases. EMT has often been used as a model for elucidating the mechanisms that underlie bladder cancer progression. However, no study to date has addressed the quantitative global variation of proteins in EMT using normal and non-malignant bladder cells. We treated normal bladder epithelial HCV29 cells and low grade nonmuscle invasive bladder cancer KK47 cells with transforming growth factor-beta (TGF-β) to establish an EMT model, and studied non-treated and treated HCV29 and KK47 cells by the stable isotope labeling amino acids in cell culture (SILAC) method. Labeled proteins were analyzed by 2D ultrahigh-resolution liquid chromatography/LTQ Orbitrap mass spectrometry. Among a total of 2994 unique identified and annotated proteins in HCV29 and KK47 cells undergoing EMT, 48 and 56 proteins, respectively, were significantly upregulated, and 106 and 24 proteins were significantly downregulated. Gene ontology (GO) term analysis and pathways analysis indicated that the differentially regulated proteins were involved mainly in enhancement of DNA maintenance and inhibition of cell-cell adhesion. Proteomes were compared for bladder cell EMT vs. bladder cancer cells, revealing 16 proteins that displayed similar changes in the two situations. Studies are in progress to further characterize these 16 proteins and their biological functions in EMT. View Full-Text
Keywords: epithelial-to-mesenchymal transition (EMT); bladder cancer; quantitative proteomics; SILAC; mass spectrometry epithelial-to-mesenchymal transition (EMT); bladder cancer; quantitative proteomics; SILAC; mass spectrometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Yang, G.; Lu, W.; Yu, D.; Sun, C.; Guo, J.; Li, Z.; Guan, F. Quantitative Analysis of Differential Proteome Expression in Epithelial-to-Mesenchymal Transition of Bladder Epithelial Cells Using SILAC Method. Molecules 2016, 21, 84.

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