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Molecules 2016, 21(1), 94; doi:10.3390/molecules21010094

HPLC Analysis, Optimization of Extraction Conditions and Biological Evaluation of Corylopsis coreana Uyeki Flos

1
Department of Oriental Medicine Materials, Dongshin University, Naju, Jeonnam 520-714, Korea
2
Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam 534-729, Korea
3
Research Develpoment Team, Jeonnam Bioindustry Foundation, Food Research Institute, Naju, Jeonnam 520-330, Korea
4
College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Korea
5
Department of Convergence Medicine, Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul 05505, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 14 December 2015 / Revised: 7 January 2016 / Accepted: 11 January 2016 / Published: 15 January 2016
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [737 KB, uploaded 15 January 2016]   |  

Abstract

A method for the separation and quantification of three flavonoids and one isocoumarin by reverse-phase high performance liquid chromatography (HPLC) has been developed and validated. Four constituents present in a crude ethanolic extract of the flowers of Coryloposis coreana Uyeki, were analyzed. Bergenin, quercetin, quercitrin and isosalipurposide were used as calibration standards. In the present study, an excellent linearity was obtained with an r2 higher than 0.999. The chromatographic peaks showed good resolution. In combination with other validation data, including precision, specificity, and accuracy, this method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of bergenin, quercetin, quercitrin and isosalipurposide in the crude ethanolic extract of C. coreana Uyeki flos. Furthermore, the plant extracts were analyzed with HPLC to determine the four constituents and compositional differences in the extracts obtained under different extraction conditions. Several extracts of them which was dependent on the ethanol percentage of solvent were also analyzed for their antimicrobial and antioxidant activities. One hundred % ethanolic extract from C. coreana Uyeki flos showed the best antimicrobial activity against the methicillin-resistant Staphylococcus aureus (MRSA) strain. Eighty % ethanolic extract showed the best antioxidant activity and phenolic content. Taken of all, these results suggest that the flower of C. coreana Uyeki flos may be a useful source for the cure and/or prevention of septic arthritis, and the validated method was useful for the quality control of C. coreana Uyeki. View Full-Text
Keywords: Corylopsis coreana Uyeki flos; simultaneous analysis; HPLC; flavonoid; isocoumarin Corylopsis coreana Uyeki flos; simultaneous analysis; HPLC; flavonoid; isocoumarin
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MDPI and ACS Style

Seo, J.-H.; Kim, J.-E.; Shim, J.-H.; Yoon, G.; Bang, M.-A.; Bae, C.-S.; Lee, K.-J.; Park, D.-H.; Cho, S.-S. HPLC Analysis, Optimization of Extraction Conditions and Biological Evaluation of Corylopsis coreana Uyeki Flos. Molecules 2016, 21, 94.

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