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Molecules 2015, 20(7), 12209-12228; doi:10.3390/molecules200712209

Qualitative and Quantitative Analysis of Phenolic Acids, Flavonoids and Iridoid Glycosides in Yinhua Kanggan Tablet by UPLC-QqQ-MS/MS

College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
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Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 6 April 2015 / Revised: 17 June 2015 / Accepted: 19 June 2015 / Published: 3 July 2015
(This article belongs to the Section Natural Products)
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Abstract

A simple, rapid and specific ultra-performance liquid chromatography-triple quadrupole mass spectrometry method was developed for the analysis of 29 bioactive components (10 phenolic acids, 16 flavonoids, and three iridoid glycosides) in Yinhua Kanggan tablet (YHKGT), a herbal prescription used for treating upper respiratory infections, fevers, coughs and pharyngalgia. The separation was successfully achieved using a Waters Cortecs UPLC C18 column (50 × 2.1 mm, 1.6 μm) and gradient elution with water-0.1% formic acid and acetonitrile. Polarity switching mode was used in the optimization of multiple reaction monitoring conditions. The analytical method was validated for linearity, precision and accuracy. Calibration curves for the 29 marker compounds showed good linear regression (r > 0.9982). The limits of detection (LOD) and limits of quantification (LOQ) for the 29 analytes were in the range of 0.03–4.99 ng/mL and 0.16–14.87 ng/mL, respectively. The relative standard deviation (RSD) values of intra-day precision, inter-day precision, repeatability, and stability were less than 2.79%, 4.87%, 4.18% and 4.71%, respectively. The recoveries of the 29 marker compounds were in the range of 94.67%–104.78% (RSD ≤ 4.72%). These results have shown that this developed method was efficient for the quality evaluation of YHKGT. View Full-Text
Keywords: Yinhua Kanggan tablet; ultra-performance liquid chromatography; triple quadrupole mass spectrometry; quality control Yinhua Kanggan tablet; ultra-performance liquid chromatography; triple quadrupole mass spectrometry; quality control
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lin, Y.; Xu, W.; Huang, M.; Xu, W.; Li, H.; Ye, M.; Zhang, X.; Chu, K. Qualitative and Quantitative Analysis of Phenolic Acids, Flavonoids and Iridoid Glycosides in Yinhua Kanggan Tablet by UPLC-QqQ-MS/MS. Molecules 2015, 20, 12209-12228.

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