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Molecules 2015, 20(5), 7890-7914; doi:10.3390/molecules20057890

A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines

1
INRA, UMR 1083 Sciences Pour l'Oenologie, Polyphenol Analysis Facility, 2 place Viala, F-34060 Montpellier, France
2
Centre de Recherche et d'Expérimentation sur le Vin Rosé, 70 avenue Wilson, F-83550 Vidauban, France
*
Author to whom correspondence should be addressed.
Academic Editors: Susan E. Ebeler and Helene Hopfer
Received: 13 March 2015 / Revised: 17 April 2015 / Accepted: 21 April 2015 / Published: 30 April 2015
(This article belongs to the Collection Wine Chemistry)
View Full-Text   |   Download PDF [964 KB, uploaded 30 April 2015]   |  

Abstract

A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, the present method allows the selective quantification of up to 152 phenolic and two additional non-phenolic wine compounds in 30 min without sample purification or pre-concentration, even at low concentration levels. This method was repeatably applied to a set of 12 rosé wines and thus proved to be suitable for high-throughput and large-scale metabolomics studies. View Full-Text
Keywords: rosé wine; polyphenols; UHPLC; Multiple Reaction Monitoring—MRM rosé wine; polyphenols; UHPLC; Multiple Reaction Monitoring—MRM
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Lambert, M.; Meudec, E.; Verbaere, A.; Mazerolles, G.; Wirth, J.; Masson, G.; Cheynier, V.; Sommerer, N. A High-Throughput UHPLC-QqQ-MS Method for Polyphenol Profiling in Rosé Wines. Molecules 2015, 20, 7890-7914.

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