Next Article in Journal
Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V
Previous Article in Journal
Anti-Inflammatory Effect of Wogonin on RAW 264.7 Mouse Macrophages Induced with Polyinosinic-Polycytidylic Acid
Article Menu

Export Article

Open AccessArticle
Molecules 2015, 20(4), 6901-6912; doi:10.3390/molecules20046901

Simultaneous Quantification of Diazepam and Dexamethasone in Plasma by High-Performance Liquid Chromatography with Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Comparison between Normoxic and Hypoxic Rats

1,2
,
3
,
2,4
,
1,3,* and 2,4,*
1
Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
2
Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
3
Department of Cognitive Sciences, Beijing Institute of Basic Medical Sciences, Beijing 100850, China
4
Beijing Laboratory for Biomedical Detection Technology and Instrument, Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 13 February 2015 / Revised: 7 April 2015 / Accepted: 13 April 2015 / Published: 16 April 2015
(This article belongs to the Section Medicinal Chemistry)
View Full-Text   |   Download PDF [938 KB, uploaded 16 April 2015]   |  

Abstract

In order to investigate the pharmacokinetics of a combination of diazepam and dexamethasone under hypoxic conditions, a novel, sensitive and specific liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of diazepam and dexamethasone in rat plasma was developed and validated. The chromatographic separation of analytes was successfully achieved on an XTerra® MS C18 column using a gradient elution of methanol and water containing 0.1% formic acid at a flow rate of 0.5 mL/min. This method demonstrated good linearity and no endogenous material interferences. The linear ranges were 1.0–100 ng/mL for diazepam and 2.0–200 ng/mL for dexamethasone. The intra- and inter-day precision for the two compounds in plasma were lower than 10.0%, and the accuracy was between −7.9% and 11.5%. Our method was then successfully applied in a pharmacokinetic comparison between normoxic and hypoxic rats. The results indicated that there were significant differences in the main pharmacokinetics parameters of diazepam and dexamethasone between normoxic and hypoxic rats. The results provide the important and valuable information for discovering and developing novel anti-hypoxia drug combinations, as well as a better understanding of the safety and efficacy of these drugs. View Full-Text
Keywords: LC-MS/MS; diazepam; dexamethasone; pharmacokinetics; hypoxia LC-MS/MS; diazepam; dexamethasone; pharmacokinetics; hypoxia
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Gong, W.; Liu, S.; Xu, P.; Fan, M.; Xue, M. Simultaneous Quantification of Diazepam and Dexamethasone in Plasma by High-Performance Liquid Chromatography with Tandem Mass Spectrometry and Its Application to a Pharmacokinetic Comparison between Normoxic and Hypoxic Rats. Molecules 2015, 20, 6901-6912.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top