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Molecules 2015, 20(4), 6913-6924; doi:10.3390/molecules20046913

Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V

1
Structural and Functional Glycobiology Unit, UMR CNRS 8576, University of Lille, 59655 Villeneuve d'Ascq, France
2
Blood Brain Barrier Laboratory (LBHE), EA 2465, University of Artois, 62300 Lens, France
3
Cell Plasticity and Cancer, INSERM U908, University of Lille, 59655 Villeneuve d'Ascq, France
Present address: Division of Biochemistry (DCH/BC), University of Natural Resources and Life Sciences, 1190 Vienna, Austria.
*
Author to whom correspondence should be addressed.
Academic Editors: Vito Ferro and Trinidad Velasco-Torrijos
Received: 14 January 2015 / Revised: 9 April 2015 / Accepted: 14 April 2015 / Published: 16 April 2015
(This article belongs to the Collection Advances in Carbohydrate Chemistry)
View Full-Text   |   Download PDF [3807 KB, uploaded 16 April 2015]   |  

Abstract

α-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid α2,6-linked to GalNAc residue that was isolated as a minor compound from the brain. The sialyltransferase ST6GalNAc V was cloned from mouse brain and showed α2,6-sialyltransferase activity almost exclusively for GM1b, to form GD1α and is considered as the main enzyme involved in the biosynthesis of α-series gangliosides. Recently, ST6GALNAC5 was identified as one of the genes over-expressed in breast cancer cell populations selected for their ability to produce brain metastasis. However, the capacity of human breast cancer cells to produce α-series gangliosides has never been clearly demonstrated. Here, we show by stable transfection and MS-MS analysis of total glycosphingolipids that ST6GALNAC5 expressing MDA-MB-231 breast cancer cells accumulate GD1α ganglioside (IV3Neu5Ac1, III6Neu5Ac1Gg4-Cer). View Full-Text
Keywords: ST6GalNAc V; breast cancer; MDA-MB-231; α-gangliosides; GD1α ST6GalNAc V; breast cancer; MDA-MB-231; α-gangliosides; GD1α
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Vandermeersch, S.; Vanbeselaere, J.; Delannoy, C.P.; Drolez, A.; Mysiorek, C.; Guérardel, Y.; Delannoy, P.; Julien, S. Accumulation of GD1α Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V. Molecules 2015, 20, 6913-6924.

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