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Molecules 2015, 20(4), 6419-6431; doi:10.3390/molecules20046419

Isolation, Identification and Cytotoxicity of a New Noroleanane-Type Triterpene Saponin from Salicornia bigelovii Torr.

1
College of Life Science, Nanjing Agricultural University, Nanjing 210095, China
2
Jiangsu Provincial Key Laboratory for Coastal Wetland Bioresources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences/Nanjing Botanical Garden Mem. Sun Yat-Sen, Nanjing 210014, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Isabel C. F. R. Ferreira
Received: 14 February 2015 / Revised: 2 April 2015 / Accepted: 3 April 2015 / Published: 10 April 2015
(This article belongs to the Collection Bioactive Compounds)
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Abstract

Salicornia bigelovii Torr. has been consumed not only as a popular kind of vegetable, but also as a medicinal plant to treat hypertension, cephalalgia, scurvy and cancer. The present study was designed to investigate its chemical components and cytotoxic activity. A new noroleanane-type triterpene saponin, bigelovii C (1), was separated and purified from Salicornia bigelovii Torr., along with four known triterpene saponins 25. The structure of bigelovii C was elucidated as 3-O-(6-O-butyl ester)-β-D-glucuropyranosyl-23-aldehyde-30-norolean-12, 20 (29)-dien-28-oic acid-28-O-β-D-glucopyranoside, according to various spectroscopic analysis and chemical characteristics. Besides Compounds 3 and 5, bigelovii C had potent cytotoxicity against three human cancer cell lines, MCF7 (breast cancer), Lovo (colon cancer) and LN229 (glioblastoma), especially MCF7. Bigelovii C inhibited the growth of MCF7 cells in dose- and time-dependent manners. Flow cytometry analysis revealed that the percentage of apoptotic cells significantly increased upon bigelovii C treatment. Rh123 staining assay indicated that bigelovii C reduced the mitochondrial membrane potential. The mechanism of cell death by bigelovii C may be attributed to the downregulation of Bcl-2 and upregulation of Bax, cleaved caspase-9, caspase-7 and PARP. These results suggested that bigelovii C may impart health benefits when consumed and should be regarded as a potential chemopreventative agent for cancer. View Full-Text
Keywords: Salicornia bigelovii Torr.; nortriterpenoid saponins; cytotoxicity; MCF-7 human breast cancer cells; apoptosis Salicornia bigelovii Torr.; nortriterpenoid saponins; cytotoxicity; MCF-7 human breast cancer cells; apoptosis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Guan, F.; Wang, Q.; Wang, M.; Shan, Y.; Chen, Y.; Yin, M.; Zhao, Y.; Feng, X.; Liu, F.; Zhang, J. Isolation, Identification and Cytotoxicity of a New Noroleanane-Type Triterpene Saponin from Salicornia bigelovii Torr.. Molecules 2015, 20, 6419-6431.

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