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Molecules 2015, 20(4), 6454-6465; doi:10.3390/molecules20046454

An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection

1
State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Avenida Wai Long, Taipa, Macau, China
2
Hunan Zhengqing Pharmaceutical Group Limited, Huaihua 418005, China
3
The First Hospital of Hunan University of Chinese Medicine, Changsha 410007, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Isabel C. F. R. Ferreira
Received: 10 March 2015 / Revised: 1 April 2015 / Accepted: 2 April 2015 / Published: 10 April 2015
(This article belongs to the Collection Bioactive Compounds)
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Abstract

A sensitive, precise and selective ultra-high performance liquid chromatography method coupled with triple-quadrupole mass spectrometry was developed and validated for the determination of trace amounts of sinomenine (ng/mL) in minute volumes of human plasma. Fifty microliter plasma samples were precipitated using methanol to extract sinomenine. Separation was carried out on a C18 column with a water and acetonitrile mobile phase gradient with formic acid as an additive. The mass spectrometry data were obtained in the positive ion mode, and the transition of multiple reactions was monitored at m/z 330.2→181.0 for sinomenine quantification. The working assay range for sinomenine was linear from 0.1173 to 15.02 ng/mL with the lower limit of quantification of 0.1173 ng/mL. The precision and accuracy of the method was less than 15% in intra-day and inter-day experiments with a matrix effect of less than 6.5%. After validation, the quantitative method was applied to analyze sinomenine levels in human plasma after transdermal delivery of the Zhengqing Fengtongning Injection. The results showed that some samples contained sinomenine within the concentration range 0.4131–4.407 ng/mL. View Full-Text
Keywords: sinomenine; Zhengqing Fengtongning Injection; transdermal delivery; human plasma; concentration; liquid chromatography-tandem mass spectrometry sinomenine; Zhengqing Fengtongning Injection; transdermal delivery; human plasma; concentration; liquid chromatography-tandem mass spectrometry
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chen, T.; Xiang, Z.; Dong, G.; Luo, P.; Qiu, P.; Wang, S.; Huang, B.; Wen, Y.; Wu, F.; Liu, L.; Zhou, H. An Ultra-High Performance Liquid Chromatographic-Tandem Mass Spectrometric Method for the Determination of Sinomenine in Human Plasma after Transdermal Delivery of the Zhengqing Fengtongning Injection. Molecules 2015, 20, 6454-6465.

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