Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry
Abstract
:1. Introduction
2. Results and Discussion
2.1. Optimization of CE-ESI-TOF/MS Methodology
2.2. Validation of the CE-TOF/MS Method
Analytes | Precision RSD, %; n = 6 | Reproducibility RSD, %; n = 6 | LOD (µg/mL) | Error (ppm) | ||
---|---|---|---|---|---|---|
Intra-Day | Inter-Day | |||||
1 | Cytosine | 8.85 | 10.97 | 22.27 | 0.25 | 1.44 |
2 | Uracil | 9.87 | 10.14 | 22.63 | 0.25 | 2.18 |
3 | Thymine | 14.56 | 7.64 | 24.34 | 0.20 | 1.54 |
4 | Adenine | 9.60 | 8.60 | 22.15 | 0.03 | 0.21 |
5 | Hypoxanthine | 8.91 | 12.26 | 19.71 | 0.12 | −0.64 |
6 | Guanine | 10.93 | 10.56 | 23.42 | 0.20 | 1.41 |
7 | Xanthine | 11.45 | 11.25 | 18.84 | 0.25 | −1.32 |
8 | Thymidine | 15.74 | 11.72 | 23.33 | 0.50 | 1.54 |
9 | Cytidine | 14.75 | ND a | ND a | 1.00 | −1.22 |
10 | Uridine | 14.85 | 10.57 | 19.00 | 0.75 | 3.21 |
11 | Cordycepin | 13.34 | 6.87 | 9.38 | 0.25 | 0.33 |
12 | Adenosine | 13.96 | 14.04 | 17.48 | 1.00 | −1.23 |
13 | 2'-Deoxyguanosine | 12.57 | 8.87 | 19.47 | 0.50 | −1.23 |
14 | Inosine | 13.79 | 16.11 | 17.32 | 0.75 | 0.20 |
15 | Guanosine | 14.58 | 8.34 | 19.52 | 0.75 | −0.86 |
2.3. Dereplication of Nucleobase and Nucleoside Compounds in Real Natural Product Samples
3. Experimental Section
3.1. Reagents, Materials and Standard Solutions
3.2. Sample Preparation
3.3. CE-ESI-TOF/MS Conditions
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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- Sample Availability: Samples of all the 15 compounds are available from the authors.
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Chen, J.; Shi, Q.; Wang, Y.; Li, Z.; Wang, S. Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry. Molecules 2015, 20, 5423-5437. https://doi.org/10.3390/molecules20045423
Chen J, Shi Q, Wang Y, Li Z, Wang S. Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry. Molecules. 2015; 20(4):5423-5437. https://doi.org/10.3390/molecules20045423
Chicago/Turabian StyleChen, Junhui, Qian Shi, Yanlong Wang, Zhaoyong Li, and Shuai Wang. 2015. "Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry" Molecules 20, no. 4: 5423-5437. https://doi.org/10.3390/molecules20045423