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Molecules 2015, 20(4), 5423-5437; doi:10.3390/molecules20045423

Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry

1
Key Lab of Analytical Technology Development and Standardization of Chinese Medicines, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
2
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
*
Author to whom correspondence should be addressed.
Academic Editor: Dilip Rai
Received: 17 December 2014 / Revised: 16 March 2015 / Accepted: 19 March 2015 / Published: 26 March 2015
(This article belongs to the Special Issue New Technologies for the Recovery of Natural Products)
View Full-Text   |   Download PDF [791 KB, uploaded 26 March 2015]   |  

Abstract

Nucleobase and nucleoside compounds exist widely in various organisms. An often occurring problem in the discovery of new bioactive compounds from natural products is reisolation of known nucleobase and nucleoside compounds. To resolve this problem, a capillary electrophoresis-high resolution mass spectrometry (CE-HR-MS) method providing both rapid separation and accurate mass full-scan MS data was developed for the first time to screen and dereplicate known nucleobase and nucleoside compounds in crude extracts of natural products. Instrumental parameters were optimized to obtain optimum conditions for CE separation and electrospray ionization-time-of-flight mass spectrometry (ESI-TOF/MS) detection. The proposed method was verified to be precise, reproducible, and sensitive. Using this method, known nucleobase and nucleoside compounds in different marine medicinal organisms including Syngnathus acus Linnaeus; Hippocampus japonicus Kaup and Anthopleura lanthogrammica Berkly were successfully observed and identified. This work demonstrates that CE-HR-MS combined with an accurate mass database may be used as a powerful tool for dereplicating known nucleobase and nucleoside compounds in different types of natural products. Rapid dereplication of known nucleobase and nucleoside compounds allows researchers to focus on other leads with greater potential to yield new substances. View Full-Text
Keywords: ESI-TOF/MS; dereplication; nucleobases; nucleosides; Syngnathus acus Linnaeus; Hippocampus japonicus Kaup; Anthopleura lanthogrammica Berkly ESI-TOF/MS; dereplication; nucleobases; nucleosides; Syngnathus acus Linnaeus; Hippocampus japonicus Kaup; Anthopleura lanthogrammica Berkly
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Chen, J.; Shi, Q.; Wang, Y.; Li, Z.; Wang, S. Dereplication of Known Nucleobase and Nucleoside Compounds in Natural Product Extracts by Capillary Electrophoresis-High Resolution Mass Spectrometry. Molecules 2015, 20, 5423-5437.

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