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Molecules 2015, 20(3), 4473-4482; doi:10.3390/molecules20034473

Inhibitory Effect of Duabanga grandiflora on MRSA Biofilm Formation via Prevention of Cell-Surface Attachment and PBP2a Production

Faculty of Science, University of Nottingham Malaysia Campus, Jalan Broga, Semenyih, Selangor 43500, Malaysia
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Author to whom correspondence should be addressed.
Academic Editor: Peter J. Rutledge
Received: 28 October 2014 / Revised: 10 December 2014 / Accepted: 12 February 2015 / Published: 10 March 2015
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Abstract

Formation of biofilms is a major factor for nosocomial infections associated with methicillin-resistance Staphylococcus aureus (MRSA). This study was carried out to determine the ability of a fraction, F-10, derived from the plant Duabanga grandiflora to inhibit MRSA biofilm formation. Inhibition of biofilm production and microtiter attachment assays were employed to study the anti-biofilm activity of F-10, while latex agglutination test was performed to study the influence of F-10 on penicillin-binding protein 2a (PBP2a) level in MRSA biofilm. PBP2a is a protein that confers resistance to beta-lactam antibiotics. The results showed that, F-10 at minimum inhibitory concentration (MIC, 0.75 mg/mL) inhibited biofilm production by 66.10%; inhibited cell-surface attachment by more than 95%; and a reduced PBP2a level in the MRSA biofilm was observed. Although ampicilin was more effective in inhibiting biofilm production (MIC of 0.05 mg/mL, 84.49%) compared to F-10, the antibiotic was less effective in preventing cell-surface attachment. A higher level of PBP2a was detected in ampicillin-treated MRSA showing the development of further resistance in these colonies. This study has shown that F-10 possesses anti-biofilm activity, which can be attributed to its ability to reduce cell-surface attachment and attenuate the level of PBP2a that we postulated to play a crucial role in mediating biofilm formation. View Full-Text
Keywords: MRSA; biofilms; Duabanga grandiflora; cell-surface attachment; PBP2a MRSA; biofilms; Duabanga grandiflora; cell-surface attachment; PBP2a
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Santiago, C.; Lim, K.-H.; Loh, H.-S.; Ting, K.N. Inhibitory Effect of Duabanga grandiflora on MRSA Biofilm Formation via Prevention of Cell-Surface Attachment and PBP2a Production. Molecules 2015, 20, 4473-4482.

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