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Molecules 2015, 20(2), 2685-2692; doi:10.3390/molecules20022685

Development and Characterization of Polymorphic Microsatellite Markers (SSRs) for an Endemic Plant, Pseudolarix amabilis (Nelson) Rehd. (Pinaceae)

1
School of Life Science, Nanjing University, Nanjing 210093, China
2
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, China
3
International Center for Tropical Botany, Department of Earth and Environment, Florida International University, Miami, FL 33199, USA
4
College of Forestry, Guangxi University, Nanning 530004, China
5
College of Life and Environment Sciences, Huangshan University, Huangshan 245041, China
6
Administration of Zhejiang Qingliangfeng National Nature Reserve, Lin'an 311321, China
These authors are equally contributed to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 14 January 2015 / Revised: 21 January 2015 / Accepted: 29 January 2015 / Published: 4 February 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [664 KB, uploaded 4 February 2015]

Abstract

Pseudolarix (Pinaceae) is a vulnerable (sensu IUCN) monotypic genus restricted to southeastern China. To better understand levels of genetic diversity, population structure and gene flow among populations of P. amabilis, we developed five compound SSR markers and ten novel polymorphic expressed sequence tags (EST) derived microsatellites. The results showed that all 15 loci were polymorphic with the number of alleles per locus ranging from two to seven. The expected and observed heterozygosities varied from 0.169 to 0.752, and 0.000 to 1.000, respectively. The inbreeding coefficient ranged from −0.833 to 1.000. These markers will contribute to research on genetic diversity and population genetic structure of P. amabilis, which in turn will contribute to the species conservation. View Full-Text
Keywords: microsatellite markers; ESTs; SSRs; Pseudolarix amabilis; Pinaceae microsatellite markers; ESTs; SSRs; Pseudolarix amabilis; Pinaceae
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Geng, Q.-F.; Liu, J.; Sun, L.; Liu, H.; Ou-Yang, Y.; Cai, Y.; Tang, X.-S.; Zhang, H.-W.; Wang, Z.-S.; An, S.-Q. Development and Characterization of Polymorphic Microsatellite Markers (SSRs) for an Endemic Plant, Pseudolarix amabilis (Nelson) Rehd. (Pinaceae). Molecules 2015, 20, 2685-2692.

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