Argentatin B Inhibits Proliferation of Prostate and Colon Cancer Cells by Inducing Cell Senescence
AbstractArgentatin B has been shown to inhibit the growth of colon HCT-15, and prostate PC-3 cancer cells. However, the mechanism by which argentatin B inhibits cell proliferation is still unknown. We aimed to investigate the mechanism by which argentatin B inhibits cell proliferation. The cell cycle was studied by flow cytometry. Apoptosis was evaluated by Annexin-V-Fluos, and Hoechst 33342 dye staining. Cell senescence was evaluated by proliferation tests, and staining for SA-β-galactosidase. Senescence-related proteins (PCNA, p21, and p27) were analyzed by Western blotting. Potential toxicity of argentatin B was evaluated in CD-1 mice. Its effect on tumor growth was tested in a HCT-15 and PC-3 xenograft model. Argentatin B induced an increment of cells in sub G1, but did not produce apoptosis. Proliferation of both cell lines was inhibited by argentatin B. Forty-three percent HCT-15, and 66% PC-3 cells showed positive SA-β-galactosidase staining. The expression of PCNA was decreased, p21 expression was increased in both cell lines, but p27 expression increased only in PC-3 cells after treatment. Administration of argentatin B to healthy mice did not produce treatment-associated pathologies. However, it restricted the growth of HCT-15 and PC-3 tumors. These results indicate that treatment with argentatin B induces cell senescence. View Full-Text
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Alcántara-Flores, E.; Brechú-Franco, A.E.; García-López, P.; Rocha-Zavaleta, L.; López-Marure, R.; Martínez-Vázquez, M. Argentatin B Inhibits Proliferation of Prostate and Colon Cancer Cells by Inducing Cell Senescence. Molecules 2015, 20, 21125-21137.
Alcántara-Flores E, Brechú-Franco AE, García-López P, Rocha-Zavaleta L, López-Marure R, Martínez-Vázquez M. Argentatin B Inhibits Proliferation of Prostate and Colon Cancer Cells by Inducing Cell Senescence. Molecules. 2015; 20(12):21125-21137.Chicago/Turabian Style
Alcántara-Flores, Ela; Brechú-Franco, Alicia E.; García-López, Patricia; Rocha-Zavaleta, Leticia; López-Marure, Rebeca; Martínez-Vázquez, Mariano. 2015. "Argentatin B Inhibits Proliferation of Prostate and Colon Cancer Cells by Inducing Cell Senescence." Molecules 20, no. 12: 21125-21137.