Next Article in Journal
Efficient Syntheses of 1,2,3-Triazoloamide Derivatives Using Solid- and Solution-Phase Synthetic Approaches
Previous Article in Journal
Water-Soluble Lignins from Different Bioenergy Crops Stimulate the Early Development of Maize (Zea mays, L.)
Article Menu

Export Article

Open AccessArticle
Molecules 2015, 20(11), 19971-19983; doi:10.3390/molecules201119670

Immobilization of Cyclooxygenase-2 on Silica Gel Microspheres: Optimization and Characterization

1
College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China
2
Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Roberto Fernandez-Lafuente
Received: 4 August 2015 / Accepted: 28 August 2015 / Published: 5 November 2015
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [2388 KB, uploaded 5 November 2015]   |  

Abstract

In this study, immobilized COX-2 was successfully constructed through glutaraldehyde-mediated covalent coupling on functional silica gel microspheres. The optimum conditions, properties, and morphological characteristics of the immobilized COX-2 were investigated. The optimal immobilization process was as follows: about 0.02 g of aminated silica gel microspheres was activated by 0.25% GA solution for 6 h and mixed with 5 U of free recombinant COX-2 solution. Then, the mixture was shaken for 8 h at 20 °C. Results showed that the immobilized COX-2 produced by this method exhibited excellent biocatalytic activity, equivalent to that of free COX-2 under the test conditions employed. The best biocatalytic activity of immobilized COX-2 appeared at pH 8.0 and still maintained at about 84% (RSD < 7.39%, n = 3) at pH 10.0. For temperature tolerance, immobilized COX-2 exhibited its maximum biocatalytic activity at 40 °C and about 68% (RSD < 6.99%, n = 3) of the activity was maintained at 60 °C. The immobilized COX-2 retained over 85% (RSD < 7.26%, n = 3) of its initial biocatalytic activity after five cycles, and after 10 days storage, the catalytic activity of immobilized COX-2 still maintained at about 95% (RSD < 3.08%, n = 3). These characteristics ensured the convenient use of the immobilized COX-2 and reduced its production cost. View Full-Text
Keywords: cyclooxyenase 2; immobilized enzyme; aminated silica gel; cross linking cyclooxyenase 2; immobilized enzyme; aminated silica gel; cross linking
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Shi, Q.; Chen, J.; Wang, Y.; Li, Z.; Li, X.; Sun, C.; Zheng, L. Immobilization of Cyclooxygenase-2 on Silica Gel Microspheres: Optimization and Characterization. Molecules 2015, 20, 19971-19983.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top