Immobilization of Cyclooxygenase-2 on Silica Gel Microspheres: Optimization and Characterization
AbstractIn this study, immobilized COX-2 was successfully constructed through glutaraldehyde-mediated covalent coupling on functional silica gel microspheres. The optimum conditions, properties, and morphological characteristics of the immobilized COX-2 were investigated. The optimal immobilization process was as follows: about 0.02 g of aminated silica gel microspheres was activated by 0.25% GA solution for 6 h and mixed with 5 U of free recombinant COX-2 solution. Then, the mixture was shaken for 8 h at 20 °C. Results showed that the immobilized COX-2 produced by this method exhibited excellent biocatalytic activity, equivalent to that of free COX-2 under the test conditions employed. The best biocatalytic activity of immobilized COX-2 appeared at pH 8.0 and still maintained at about 84% (RSD < 7.39%, n = 3) at pH 10.0. For temperature tolerance, immobilized COX-2 exhibited its maximum biocatalytic activity at 40 °C and about 68% (RSD < 6.99%, n = 3) of the activity was maintained at 60 °C. The immobilized COX-2 retained over 85% (RSD < 7.26%, n = 3) of its initial biocatalytic activity after five cycles, and after 10 days storage, the catalytic activity of immobilized COX-2 still maintained at about 95% (RSD < 3.08%, n = 3). These characteristics ensured the convenient use of the immobilized COX-2 and reduced its production cost. View Full-Text
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Shi, Q.; Chen, J.; Wang, Y.; Li, Z.; Li, X.; Sun, C.; Zheng, L. Immobilization of Cyclooxygenase-2 on Silica Gel Microspheres: Optimization and Characterization. Molecules 2015, 20, 19971-19983.
Shi Q, Chen J, Wang Y, Li Z, Li X, Sun C, Zheng L. Immobilization of Cyclooxygenase-2 on Silica Gel Microspheres: Optimization and Characterization. Molecules. 2015; 20(11):19971-19983.Chicago/Turabian Style
Shi, Qian; Chen, Junhui; Wang, Yanlong; Li, Zhaoyong; Li, Xianguo; Sun, Chengjun; Zheng, Li. 2015. "Immobilization of Cyclooxygenase-2 on Silica Gel Microspheres: Optimization and Characterization." Molecules 20, no. 11: 19971-19983.