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Molecules 2014, 19(9), 14114-14127; doi:10.3390/molecules190914114

RP-HPLC Characterization of Lupenone and β-Sitosterol in Rhizoma Musae and Evaluation of the Anti-Diabetic Activity of Lupenone in Diabetic Sprague-Dawley Rats

Department of Pharmacognosy, Guiyang College of Traditional Chinese Medicine, 50, Nanming District, Guiyang 550002, Guizhou, China
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Received: 23 June 2014 / Revised: 17 August 2014 / Accepted: 1 September 2014 / Published: 9 September 2014
(This article belongs to the Section Medicinal Chemistry)
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Abstract

With the aim of characterizing the active ingredients lupenone and β-sitosterol in Rhizoma Musae samples a reversed-phase HPLC method for the separation of these two compounds in Rhizoma Musae samples was developed (regression coefficient > 0.9996). The method was further applied to quantify lupenone and β-sitosterol content in Rhizoma Musae samples cultured in different growth environments. Different variables such as geographical location, growth stage, and harvest time, demonstrated differential effects on lupenone and β-sitosterol levels. Moreover, we determined the optimum conditions for cultivation and harvesting of Rhizoma Musae herbs. Lupenone administration caused a significant reduction in fasting blood glucose (FBG) levels in diabetic rats at doses of 1.78, 5.33, and 16.00 mg·kg−1·day−1 for 14 days, the glycated hemoglobin (HbA1c) levels of diabetic rats also significantly reduced at doses of 5.33, and 16.00 mg·kg−1·day−1, indicating a robust antidiabetic activity. To our knowledge, this is the first report of an optimized HPLC method successfully applied to quantify lupenone and β-sitosterol, and its applicability in optimizing Rhizoma Musae growth. Animal experiments also showed for the first time that lupenone from Rhizoma Musae has anti-diabetic activity. View Full-Text
Keywords: Rhizoma Musae; diabetes; lupenone; β-sitosterol; HbA1c; cultivation and harvesting Rhizoma Musae; diabetes; lupenone; β-sitosterol; HbA1c; cultivation and harvesting
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Xu, F.; Wu, H.; Wang, X.; Yang, Y.; Wang, Y.; Qian, H.; Zhang, Y. RP-HPLC Characterization of Lupenone and β-Sitosterol in Rhizoma Musae and Evaluation of the Anti-Diabetic Activity of Lupenone in Diabetic Sprague-Dawley Rats. Molecules 2014, 19, 14114-14127.

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