Headspace Solid-Phase Microextraction Analysis of Volatile Components in Phalaenopsis Nobby’s Pacific Sunset
Abstract
:1. Introduction
2. Results and Discussion
2.1. Comparison Optimal of SPME Fiber Coating
2.2. Analysis of the Volatiles in P. Nobby’s Pacific Sunset Flowers
RI a | Compound | Content (%) |
---|---|---|
641 | 2-methylbutanal | 0.60 ± 0.15 |
882 | styrene | 0.02 ± 0.00 |
933 | benzaldehyde | 0.08 ± 0.02 |
964 | 6-methyl-5-hepten-2-one | 0.69 ± 0.55 |
981 | β-myrcene | 3.87 ± 0.18 |
1021 | limonene | 1.30 ± 0.04 |
1023 | cis-β-ocimene | 2.17 ± 0.29 |
1035 | trans-β-ocimene | 0.07 ± 0.02 |
1050 | α-ocimene | 0.10 ± 0.01 |
1060 | 3-methylphenol | 0.07 ± 0.02 |
1065 | 2-methylphenol | 0.22 ± 0.10 |
1080 | linalool | 28.30 ± 16.78 |
1099 | (E)-4,8-dimethyl-1,3,7-nonatriene | 0.82 ± 0.08 |
1189 | methyl salicylate | 0.07 ± 0.02 |
1201 | nerol | 4.54 ± 1.05 |
1231 | geraniol | 28.31 ± 8.97 |
1233 | geranial | 2.04 ± 0.17 |
1357 | neryl acetate | 0.91 ± 0.57 |
1360 | geranyl acetate | 0.52 ± 0.26 |
1444 | trans-β-farnesene | 1.08 ± 0.87 |
1469 | α-humulene | 0.17 ± 0.13 |
1477 | α-curcumene | 0.29 ± 0.19 |
1481 | (Z,E)-α-farnesene | 0.47 ± 0.07 |
1489 | α-zingiberene | 0.20 ± 0.10 |
1490 | cis-γ-bisabolene | <0.01 |
1495 | (E,E)-α-farnesene | 11.10 ± 4.49 |
1544 | nerolidol | 0.31 ± 0.25 |
1556 | trans-γ-bisabolene | 0.17 ± 0.04 |
1686 | cis-11-hexadecenal | 0.10 ± 0.07 |
1705 | (Z,E)-farnesol | 1.18 ± 0.82 |
1721 | (E,E)-farnesal | 0.17 ± 0.15 |
Total | 89.91 ± 1.19 |
2.2.1. Volatile Compounds of P. Nobby’s Pacific Sunset Flowers over a 24 h Period
2.2.2. Volatile Compounds of P. Nobby’s Pacific Sunset Flowers in a Blooming Cycle
2.3. Volatile Compounds of Different Parts of the P. Nobby’s Pacific Sunset Flowers
RI a | Compound | Whole Flowers | Petals | Lateral Sepals | Dorsal Sepals | Lip | Column |
---|---|---|---|---|---|---|---|
641 | 2-methyl butanal | 0.60 ± 0.15 | 0.26 ± 0.24 | - b | - | 0.17 ± 0.12 | - |
882 | styrene | 0.02 ± 0.00 | 1.28 ± 1.16 | 0.86 ± 0.62 | 2.80 ± 1.78 | - | - |
933 | benzaldehyde | 0.08 ± 0.02 | - | 0.07 ± 0.00 | 0.07 ± 0.00 | 0.05 ± 0.02 | - |
964 | 6-methyl-5-hepten-2-one | 0.69 ± 0.55 | - | 0.48 ± 0.24 | - | - | - |
981 | β-myrcene | 3.87 ± 0.18 | 6.83 ± 0.05 | 6.35 ± 0.46 | 5.93 ± 0.45 | 5.62 ± 1.33 | 4.89 ± 0.21 |
1021 | limonene | 1.30 ± 0.04 | 2.27 ± 0.02 | 2.12 ± 0.34 | - | 1.95 ± 0.39 | - |
1023 | cis-β-ocimene | <0.01 | - | - | - | - | 2.39 ± 0.32 |
1035 | trans-β-ocimene | 2.16 ± 0.28 | 3.60 ± 1.03 | 2.98 ± 0.22 | 3.21 ± 0.65 | 2.53 ± 0.68 | 6.44 ± 1.62 |
1050 | α-ocimene | 0.07 ± 0.02 | 0.10 ± 0.01 | 0.10 ± 0.03 | 0.08 ± 0.02 | 0.12 ± 0.01 | 0.35 ± 0.02 |
1060 | 3-methylphenol | 0.07 ± 0.02 | 0.10 ± 0.01 | - | - | - | - |
1065 | 2-methylphenol | 0.22 ± 0.10 | 0.33 ± 0.02 | 0.32 ± 0.13 | 0.29 ± 0.06 | 0.53 ± 0.06 | 0.84 ± 0.24 |
1080 | linalool | 28.30 ± 16.78 | 40.24 ± 2.77 | 39.53 ± 7.40 | 42.04 ± 3.08 | 67.77 ± 4.98 | 63.53 ± 6.57 |
1099 | (E)-4,8-dimethyl-1,3,7-nonatriene | 0.51 ± 0.06 | 1.33 ± 0.15 | 1.20 ± 0.13 | 1.90 ± 0.29 | 0.80 ± 0.05 | - |
1189 | methyl salicylate | 0.11 ± 0.05 | - | - | - | - | - |
1201 | nerol | 4.54 ± 1.05 | 2.40 ± 0.77 | - | - | - | - |
1231 | geraniol | 28.31 ± 8.97 | 15.68 ± 4.39 | 17.77 ± 6.43 | 9.88 ± 2.07 | 6.50 ± 1.92 | - |
1233 | geranial | - | 1.15 ± 0.12 | 1.17 ± 0.15 | - | - | - |
1357 | neryl acetate | 0.91 ± 0.57 | - | - | - | 0.59 ± 0.53 | - |
1360 | geranyl acetate | 0.52 ± 0.26 | - | - | - | - | - |
1444 | trans-β-farnesene | 1.08 ± 0.87 | - | - | - | - | - |
1469 | α-humulene | 0.27 ± 0.07 | - | - | - | - | - |
1477 | α-curcumene | 0.29 ± 0.19 | 0.48 ± 0.16 | 0.46 ± 0.08 | 0.57 ± 0.11 | 0.16 ± 0.03 | - |
1481 | (Z,E)-α-farnesene | 0.47 ± 0.07 | - | - | - | - | - |
1489 | α-zingiberene | 0.20 ± 0.10 | - | - | - | - | - |
1490 | cis-γ-bisabolene | <0.01 | - | - | - | - | - |
1495 | (E,E)-α-farnesene | 11.10 ± 4.49 | 10.33 ± 1.27 | 10.71 ± 3.10 | 13.73 ± 1.32 | 2.59 ± 0.47 | 4.83 ± 3.08 |
1544 | nerolidol | 0.31 ± 0.24 | - | - | - | - | - |
1556 | trans-γ-bisabolene | 0.17 ± 0.05 | - | - | - | - | - |
1686 | cis-11-hexadecenal | 0.19 ± 0.05 | - | 0.03 ± 0.03 | - | - | - |
1705 | (Z,E)-farnesol | 0.82 ± 0.85 | - | - | - | - | - |
1721 | (E,E)-farnesal | 0.17 ± 0.15 | - | - | - | - | - |
3. Experimental Section
3.1. Plant Materials
3.2. Methods
3.2.1. Comparison of SPME Fiber Coating
3.2.2. Volatile Components of P. Nobby’s Pacific Sunset
3.2.3. Analysis of Volatile Compounds
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Yeh, C.-H.; Tsai, W.-Y.; Chiang, H.-M.; Wu, C.-S.; Lee, Y.-I.; Lin, L.-Y.; Chen, H.-C. Headspace Solid-Phase Microextraction Analysis of Volatile Components in Phalaenopsis Nobby’s Pacific Sunset. Molecules 2014, 19, 14080-14093. https://doi.org/10.3390/molecules190914080
Yeh C-H, Tsai W-Y, Chiang H-M, Wu C-S, Lee Y-I, Lin L-Y, Chen H-C. Headspace Solid-Phase Microextraction Analysis of Volatile Components in Phalaenopsis Nobby’s Pacific Sunset. Molecules. 2014; 19(9):14080-14093. https://doi.org/10.3390/molecules190914080
Chicago/Turabian StyleYeh, Chih-Hsin, Wan-Yu Tsai, Hsiu-Mei Chiang, Chin-Sheng Wu, Yung-I Lee, Li-Yun Lin, and Hsin-Chun Chen. 2014. "Headspace Solid-Phase Microextraction Analysis of Volatile Components in Phalaenopsis Nobby’s Pacific Sunset" Molecules 19, no. 9: 14080-14093. https://doi.org/10.3390/molecules190914080