Next Article in Journal
Synthesis, Leishmanicidal and Cytotoxic Activity of Triclosan-Chalcone, Triclosan-Chromone and Triclosan-Coumarin Hybrids
Previous Article in Journal
Cytotoxic Compounds Isolated from Murraya tetramera Huang
Article Menu

Export Article

Correction published on 20 May 2016, see Molecules 2016, 21(5), 673.

Open AccessArticle
Molecules 2014, 19(9), 13235-13250; doi:10.3390/molecules190913235

Schisandrin B Induces Apoptosis and Cell Cycle Arrest of Gallbladder Cancer Cells

1,2,3,†, 1,2,3,†, 1,2,3,†, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 4,* and 1,2,3,*
1
Department of General Surgery, School of Medicine, Shanghai Jiao Tong University, No. 1665 Kongjiang Road, Shanghai 200092, China
2
Laboratory of General Surgery, School of Medicine, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, No. 1665 Kongjiang Road, Shanghai 200092, China
3
Institute of Biliary Tract Disease, School of Medicine, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University, No. 1665 Kongjiang Road, Shanghai 200092, China
4
The First Affiliated Hospital Nanchang University Emergency Unit, No. 17 Yongwai Road, Nanchang 330006, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 29 July 2014 / Revised: 13 August 2014 / Accepted: 18 August 2014 / Published: 27 August 2014
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [4185 KB, uploaded 20 May 2016]   |  

Abstract

Gallbladder cancer, with high aggressivity and extremely poor prognosis, is the most common malignancy of the bile duct. The main objective of the paper was to investigate the effects of schisandrin B (Sch B) on gallbladder cancer cells and identify the mechanisms underlying its potential anticancer effects. We showed that Sch B inhibited the viability and proliferation of human gallbladder cancer cells in a dose-, time -dependent manner through MTT and colony formation assays, and decrease mitochondrial membrane potential (ΔΨm) at a dose-dependent manner through flow cytometry. Flow cytometry assays also revealed G0/G1 phase arrest and apoptosis in GBC-SD and NOZ cells. Western blot analysis of Sch B-treated cells revealed the upregulation of Bax, cleaved caspase-9, cleaved caspase-3, cleaved PARP and downregulation of Bcl-2, NF-κB, cyclin D1 and CDK-4. Moreover, this drug also inhibited the tumor growth in nude mice carrying subcutaneous NOZ tumor xenografts. These data demonstrated that Sch B induced apoptosis in gallbladder cancer cells by regulating apoptosis-related protein expression, and suggests that Sch B may be a promising drug for the treatment of gallbladder cancer. View Full-Text
Keywords: schisandrin B; gallbladder cancer; apoptosis; cell cycle arrest; mitochondrial pathway schisandrin B; gallbladder cancer; apoptosis; cell cycle arrest; mitochondrial pathway
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Xiang, S.-S.; Wang, X.-A.; Li, H.-F.; Shu, Y.-J.; Bao, R.-F.; Zhang, F.; Cao, Y.; Ye, Y.-Y.; Weng, H.; Wu, W.-G.; Mu, J.-S.; Wu, X.-S.; Li, M.-L.; Hu, Y.-P.; Jiang, L.; Tan, Z.-J.; Lu, W.; Liu, F.; Liu, Y.-B. Schisandrin B Induces Apoptosis and Cell Cycle Arrest of Gallbladder Cancer Cells. Molecules 2014, 19, 13235-13250.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top