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Molecules 2014, 19(8), 11145-11159; doi:10.3390/molecules190811145

Effects of Tatariside G Isolated from Fagopyrum tataricum Roots on Apoptosis in Human Cervical Cancer HeLa Cells

1
Department of Chinese Materia Medica, School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, China
2
Central Laboratory, Shanghai Seventh People's Hospital, Shanghai 200137, China
3
Department of Nuclear Medicine, Shanghai Seventh People's Hospital, Shanghai 200137, China
4
School of Pharmacy and Biomolecular Sciences, Faculty of Science, Liverpool John Moores University, Liverpool L3 3AF, England, UK
5
Department of Pharmaceutical Botany, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
6
Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
*
Authors to whom correspondence should be addressed.
Received: 21 June 2014 / Revised: 11 July 2014 / Accepted: 16 July 2014 / Published: 29 July 2014
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1261 KB, uploaded 29 July 2014]   |  

Abstract

Cervical cancer is the second most common female carcinoma. Current therapies are often unsatisfactory, especially for advanced stage patients. The aim of this study was to explore the effects of tatariside G (TG) on apoptosis in human cervical cancer HeLa cells and the possible mechanism of action involved. An MTT assay was employed to evaluate cell viability. Hoechst 33258 staining and flow cytometry (FCM) assays were used to detect cell apoptosis. The protein expression of phosphorylated JNK, P38, ERK and Akt and cleaved caspase-3 and caspase-9 was evaluated by western blot analysis. Additionally, the mRNA expression of caspase-3 and caspase-9 was measured by fluorescent quantitative reverse transcription-PCR (FQ-RT-PCR). TG notably inhibited cell viability, enhanced the percentage of apoptotic cells, facilitated the phosphorylation of p38 MAPK and JNK proteins and caspase-3 and caspase-9 cracking, downregulated the phosphorylation level of Akt, and increased the loss of MMP and the mRNA expression of caspase-3 and caspase-9. TG-induced apoptosis is associated with activation of the mitochondrial death pathway. TG may be an effective candidate for chemotherapy against cervical cancer. View Full-Text
Keywords: natural product; cervical cancer; apoptosis; mitochondria; caspase natural product; cervical cancer; apoptosis; mitochondria; caspase
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Li, Y.; Wang, S.-J.; Xia, W.; Rahman, K.; Zhang, Y.; Peng, H.; Zhang, H.; Qin, L.-P. Effects of Tatariside G Isolated from Fagopyrum tataricum Roots on Apoptosis in Human Cervical Cancer HeLa Cells. Molecules 2014, 19, 11145-11159.

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