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Molecules 2014, 19(8), 11131-11144; https://doi.org/10.3390/molecules190811131

Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production

Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
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Received: 9 May 2014 / Revised: 14 July 2014 / Accepted: 21 July 2014 / Published: 29 July 2014
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Abstract

The biofloculant production potential of a consortium of Streptomyces and Brachybacterium species were evaluated. Optimum bioflocculant yields (g/L) and flocculation activities (%) were observed for the following preferred nutritional sources: glucose (56%; 2.78 ± 0.15 g/L), (NH4)2NO3 (53%; 2.81 ± 0.37 g/L) and CaSO4·H2O (47%; 2.19 ± 0.13 g/L). A Plackett-Burman design revealed the critical fermentation media components. The concentrations of these components were optimized [glucose; 16.0, (NH4)2NO3; 0.5 and CaSO4·H2O; 1.2 (g/L)] through a central composite design with optimum bioflocculant yield of 3.02 g/L and flocculation activity of 63.7%. The regression coefficient (R2 = 0.6569) indicates a weak estimation of the model’s adequacy and a high lack-of-fit value (34.1%). Lack of synergy in the consortium may have been responsible for the model inadequacy observed. FTIR spectrometry showed the bioflocculant to be a heteropolysaccharide, while SEM imaging revealed an amorphous loosely arranged fluffy structure with interstial spacing of less than 1 µm. View Full-Text
Keywords: bioflocculant; Brachybacterium; mixed culture; optimization; Streptomyces bioflocculant; Brachybacterium; mixed culture; optimization; Streptomyces
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Nwodo, U.U.; Okoh, A.I. Mixed Culture Fermentation and Media Optimization by Response Surface Model: Streptomyces and Brachybacterium Species in Bioflocculant Production. Molecules 2014, 19, 11131-11144.

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