Molecules 2014, 19(7), 10563-10573; doi:10.3390/molecules190710563
Article

Isolation and Characterization of an α-Glucosidase Inhibitor from Musa spp. (Baxijiao) Flowers

1,2email, 3,* email, 1email, 4email, 4email and 2,* email
Received: 3 June 2014; in revised form: 15 July 2014 / Accepted: 15 July 2014 / Published: 18 July 2014
(This article belongs to the collection Bioactive Compounds)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. Using a bioassay-guided fractionation technique, five Bacillus stearothermophilus α-glucosidase inhibitors were isolated from the flowers of Musa spp. (Baxijiao). Using NMR spectroscopy analysis they were identified as vanillic acid (1), ferulic acid (2), β-sitosterol (3), daucosterol (4) and 9-(4′-hydroxyphenyl)-2-methoxyphenalen-1-one (5). The half maximal inhibitory concentration (IC50) values of compounds 15 were 2004.58, 1258.35, 283.67, 247.35 and 3.86 mg/L, respectively. Compared to a known α-glucosidase inhibitor (acarbose, IC50 = 999.31 mg/L), compounds 3, 4 and 5 showed a strong α-glucosidase inhibitory effect. A Lineweaver-Burk plot indicated that compound 5 is a mixed-competitive inhibitor, while compounds 3 and 4 are competitive inhibitors. The inhibition constants (Ki) of compounds 3, 4 and 5 were 20.09, 2.34 and 4.40 mg/L, respectively. Taken together, these data show that the compounds 3, 4 and 5 are potent α-glucosidase inhibitors.
Keywords: α-glucosidase; inhibitor; hyperglycemia; flowers of Musa spp. (Baxijiao)
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MDPI and ACS Style

Sheng, Z.; Dai, H.; Pan, S.; Wang, H.; Hu, Y.; Ma, W. Isolation and Characterization of an α-Glucosidase Inhibitor from Musa spp. (Baxijiao) Flowers. Molecules 2014, 19, 10563-10573.

AMA Style

Sheng Z, Dai H, Pan S, Wang H, Hu Y, Ma W. Isolation and Characterization of an α-Glucosidase Inhibitor from Musa spp. (Baxijiao) Flowers. Molecules. 2014; 19(7):10563-10573.

Chicago/Turabian Style

Sheng, Zhanwu; Dai, Haofu; Pan, Siyi; Wang, Hui; Hu, Yingying; Ma, Weihong. 2014. "Isolation and Characterization of an α-Glucosidase Inhibitor from Musa spp. (Baxijiao) Flowers." Molecules 19, no. 7: 10563-10573.

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