Molecules 2014, 19(6), 7629-7645; doi:10.3390/molecules19067629
Article

Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases

1email, 2email, 3email, 1,4email, 5email and 1,* email
Received: 3 April 2014; in revised form: 3 June 2014 / Accepted: 4 June 2014 / Published: 10 June 2014
(This article belongs to the Special Issue Enzyme Immobilization)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A commercial and very hydrophobic styrene-divinylbenzene matrix, MCI GEL® CHP20P, has been compared to octyl-Sepharose® beads as support to immobilize three different enzymes: lipases from Thermomyces lanuginosus (TLL) and from Rhizomucor miehie (RML) and Lecitase® Ultra, a commercial artificial phospholipase. The immobilization mechanism on both supports was similar: interfacial activation of the enzymes versus the hydrophobic surface of the supports. Immobilization rate and loading capacity is much higher using MCI GEL® CHP20P compared to octyl-Sepharose® (87.2 mg protein/g of support using TLL, 310 mg/g using RML and 180 mg/g using Lecitase® Ultra). The thermal stability of all new preparations is much lower than that of the standard octyl-Sepharose® immobilized preparations, while the opposite occurs when the inactivations were performed in the presence of organic co-solvents. Regarding the hydrolytic activities, the results were strongly dependent on the substrate and pH of measurement. Octyl-Sepharose® immobilized enzymes were more active versus p-NPB than the enzymes immobilized on MCI GEL® CHP20P, while RML became 700-fold less active versus methyl phenylacetate. Thus, the immobilization of a lipase on this matrix needs to be empirically evaluated, since it may present very positive effects in some cases while in other cases it may have very negative ones.
Keywords: lipase immobilization, modulation of lipase activity, interfacial activation, styrene divinylbencene matrix
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MDPI and ACS Style

Garcia-Galan, C.; Barbosa, O.; Hernandez, K.; Santos, J.C.S.; Rodrigues, R.C.; Fernandez-Lafuente, R. Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases. Molecules 2014, 19, 7629-7645.

AMA Style

Garcia-Galan C, Barbosa O, Hernandez K, Santos JCS, Rodrigues RC, Fernandez-Lafuente R. Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases. Molecules. 2014; 19(6):7629-7645.

Chicago/Turabian Style

Garcia-Galan, Cristina; Barbosa, Oveimar; Hernandez, Karel; Santos, Jose C.S.; Rodrigues, Rafael C.; Fernandez-Lafuente, Roberto. 2014. "Evaluation of Styrene-Divinylbenzene Beads as a Support to Immobilize Lipases." Molecules 19, no. 6: 7629-7645.


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