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Molecules 2014, 19(6), 7189-7206; doi:10.3390/molecules19067189

Effects of the Total Saponins from Rosa laevigata Michx Fruit against Acetaminophen-Induced Liver Damage in Mice via Induction of Autophagy and Suppression of Inflammation and Apoptosis

1
College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Lvshunkou District, Dalian 116044, Liaoning Province, China
2
The First Affiliated Hospital of Dalian Medical University, Dalian 116011, China
3
Research Institute of Integrated Traditional and Western Medicine of Dalian Medical University, Dalian 116011, China
*
Author to whom correspondence should be addressed.
Received: 4 May 2014 / Revised: 23 May 2014 / Accepted: 25 May 2014 / Published: 30 May 2014
(This article belongs to the Section Natural Products)
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Abstract

The effect of the total saponins from Rosa laevigata Michx fruit (RLTS) against acetaminophen (APAP)-induced liver damage in mice was evaluated in the present paper. The results showed that RLTS markedly improved the levels of liver SOD, CAT, GSH, GSH-Px, MDA, NO and iNOS, and the activities of serum ALT and AST caused by APAP. Further research confirmed that RLTS prevented fragmentation of DNA and mitochondrial ultrastructural alterations based on TdT-mediated dUTP nick end labeling (TUNEL) and transmission electron microscopy (TEM) assays. In addition, RLTS decreased the gene or protein expressions of cytochrome P450 (CYP2E1), pro-inflammatory mediators (IL-1β, IL-4, IL-6, TNF-α, iNOS, Bax, HMGB-1 and COX-2), pro-inflammatory transcription factors (NF-κB and AP-1), pro-apoptotic proteins (cytochrome C, p53, caspase-3, caspase-9, p-JNK, p-p38 and p-ERK), and increased the protein expressions of Bcl-2 and Bcl-xL. Moreover, the gene expression of IL-10, and the proteins including LC3, Beclin-1 and Atg5 induced by APAP were even more augmented by the extract. These results demonstrate that RLTS has hepatoprotective effects through antioxidative action, induction of autophagy, and suppression of inflammation and apoptosis, and could be developed as a potential candidate to treat APAP-induced liver damage in the future. View Full-Text
Keywords: acetaminophen; hepatoprotective effect; liver injury; Rosa laevigata Michx; total saponins acetaminophen; hepatoprotective effect; liver injury; Rosa laevigata Michx; total saponins
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MDPI and ACS Style

Dong, D.; Xu, L.; Han, X.; Qi, Y.; Xu, Y.; Yin, L.; Liu, K.; Peng, J. Effects of the Total Saponins from Rosa laevigata Michx Fruit against Acetaminophen-Induced Liver Damage in Mice via Induction of Autophagy and Suppression of Inflammation and Apoptosis. Molecules 2014, 19, 7189-7206.

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