miR-221/222 Promotes S-Phase Entry and Cellular Migration in Control of Basal-Like Breast Cancer
AbstractThe miR-221/222 cluster has been demonstrated to function as oncomiR in human cancers. miR-221/222 promotes epithelial-to-mesenchymal transition (EMT) and confers tamoxifen resistance in breast cancer. However, the effects and mechanisms by which miR-221/222 regulates breast cancer aggressiveness remain unclear. Here we detected a much higher expression of miR-221/222 in highly invasive basal-like breast cancer (BLBC) cells than that in non-invasive luminal cells. A microRNA dataset from breast cancer patients indicated an elevated expression of miR-221/222 in BLBC subtype. S-phase entry of the cell cycle was associated with the induction of miR-221/222 expression. miRNA inhibitors specially targeting miR-221 or miR-222 both significantly suppressed cellular migration, invasion and G1/S transition of the cell cycle in BLBC cell types. Proteomic analysis demonstrated the down-regulation of two tumor suppressor genes, suppressor of cytokine signaling 1 (SOCS1) and cyclin-dependent kinase inhibit 1B (CDKN1B), by miR-221/222. This is the first report to reveal miR-221/222 regulation of G1/S transition of the cell cycle. These findings demonstrate that miR-221/222 contribute to the aggressiveness in control of BLBC. View Full-Text
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Li, Y.; Liang, C.; Ma, H.; Zhao, Q.; Lu, Y.; Xiang, Z.; Li, L.; Qin, J.; Chen, Y.; Cho, W.C.; Pestell, R.G.; Liang, L.; Yu, Z. miR-221/222 Promotes S-Phase Entry and Cellular Migration in Control of Basal-Like Breast Cancer. Molecules 2014, 19, 7122-7137.
Li Y, Liang C, Ma H, Zhao Q, Lu Y, Xiang Z, Li L, Qin J, Chen Y, Cho WC, Pestell RG, Liang L, Yu Z. miR-221/222 Promotes S-Phase Entry and Cellular Migration in Control of Basal-Like Breast Cancer. Molecules. 2014; 19(6):7122-7137.Chicago/Turabian Style
Li, Yuan; Liang, Chunli; Ma, Haizhong; Zhao, Qian; Lu, Ying; Xiang, Zhendong; Li, Li; Qin, Jie; Chen, Yihan; Cho, William C.; Pestell, Richard G.; Liang, Li; Yu, Zuoren. 2014. "miR-221/222 Promotes S-Phase Entry and Cellular Migration in Control of Basal-Like Breast Cancer." Molecules 19, no. 6: 7122-7137.