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Molecules 2014, 19(4), 5448-5458; doi:10.3390/molecules19045448
Article

Two Ellagic Acids Isolated from Roots of Sanguisorba officinalis L. Promote Hematopoietic Progenitor Cell Proliferation and Megakaryocyte Differentiation

1,†,* , 1,2,†
, 1,*  and 1
Received: 7 March 2014; in revised form: 18 April 2014 / Accepted: 22 April 2014 / Published: 24 April 2014
(This article belongs to the Section Metabolites)
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Abstract: Using a bioassay-directed chromatographic separation, two ellagic acids were obtained from the ethyl acetate extract of the roots of Sanguisorba officinalis L. On the basis of chemical and spectroscopic methods, the two ellagic acids were identified as 3,3',4-tri-O-methylellagic acid-4'-O-β-d-xyloside and 3,3',4-tri-O-methylellagic acid. Stimulation of cell proliferation was assayed in hematopoietic progenitor cells using the Cell Counting kit-8 method. The megakaryocyte differentiation was determined in human erythroleukemia (HEL) cells using Giemsa staining and flow cytometry analysis. The ellagic acids significantly stimulated the proliferation of Baf3/Mpl cells. Morphology analysis and megakaryocyte specific-marker CD41 staining confirmed that the ellagic acids induced megakaryocyte differentiation in HEL cells. This is the first time that 3,3',4-tri-O-methylellagic acid or 3,3',4-tri-O-methylellagic acid-4'-O-β-d-xyloside are reported to induce megakaryopoiesis, suggesting a class of small molecules which differ from others non-peptidyl, and appears to have potential for clinical development as a therapeutic agent for patients with blood platelet disorders.
Keywords: Sanguisorba officinalis L; 3,3',4-tri-O-methylellagic acid 4'-O-β-d-xyloside; 3,3',4-tri-O-methylellagic acid; hematopoietic progenitor cells; cell proliferation; megakaryocyte differentiation Sanguisorba officinalis L; 3,3',4-tri-O-methylellagic acid 4'-O-β-d-xyloside; 3,3',4-tri-O-methylellagic acid; hematopoietic progenitor cells; cell proliferation; megakaryocyte differentiation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Gao, X.; Wu, J.; Zou, W.; Dai, Y. Two Ellagic Acids Isolated from Roots of Sanguisorba officinalis L. Promote Hematopoietic Progenitor Cell Proliferation and Megakaryocyte Differentiation. Molecules 2014, 19, 5448-5458.

AMA Style

Gao X, Wu J, Zou W, Dai Y. Two Ellagic Acids Isolated from Roots of Sanguisorba officinalis L. Promote Hematopoietic Progenitor Cell Proliferation and Megakaryocyte Differentiation. Molecules. 2014; 19(4):5448-5458.

Chicago/Turabian Style

Gao, Xiaoping; Wu, Jianming; Zou, Wenjun; Dai, Yanping. 2014. "Two Ellagic Acids Isolated from Roots of Sanguisorba officinalis L. Promote Hematopoietic Progenitor Cell Proliferation and Megakaryocyte Differentiation." Molecules 19, no. 4: 5448-5458.



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